Huang, Pung-LingPung-LingHuangDo, Yi-YinYi-YinDoChana, H.T.H.T.ChanaHuang, P.L.P.L.HuangChan, T.S.T.S.ChanRU-SHI LIUHuang, C.Y.C.Y.HuangYang, S.Y.S.Y.YangHorng, H.E.H.E.Horng2010-05-062018-06-292010-05-062018-06-292006http://www.scopus.com/inward/record.url?eid=2-s2.0-33646863030&partnerID=MN8TOARShttp://ntur.lib.ntu.edu.tw//handle/246246/181300http://ntur.lib.ntu.edu.tw/bitstream/246246/181300/1/32.pdfIn this work, we study the preparation and properties of bio-compatible magnetic nanoparticles for immunoassay and DNA detection. The magnetite (Fe3O4) nanoparticles were prepared by a chemical co-precipitation method and dextran was selected as the surfactant to suspend the nanoparticles. Suspended particles associated with avidin followed by biotin were qualitatively analyzed by enzyme-linked immunosorbent assay (ELISA) method. We found further the ethylenediamine blocked activated residual groups efficiently, hence enhancing the attachment of biotin for probing the avidin. © 2006 Elsevier B.V. All rights reserved.application/pdf191098 bytesapplication/pdfen-USAvidin; Bio-compatible nanoparticles; Biotinylated alkaline phosphatase; Hybridizationjournal article10.1016/j.jmmm.2006.01.1262-s2.0-33646863030http://ntur.lib.ntu.edu.tw/bitstream/246246/181300/1/32.pdf