National Taiwan University Dept ChemLin, Ching-ErhChing-ErhLinChen, Kuo-HsingKuo-HsingChenHsiao, Yu-YouYu-YouHsiaoLiao, Wei-SsuWei-SsuLiaoChen, Chia-ChongChia-ChongChen2006-11-152018-07-102006-11-152018-07-102002http://ntur.lib.ntu.edu.tw//handle/246246/2006111501233146In this study, enantioseparations of five phenothiazines in cyclodextrin (CD)-modified micellar electrokinetic chromatography (MEKC) were investigated using a citrate buffer containing tetradecyltrimethylammonium bromide (TTAB) as a cationic surfactant at low pH. b-Cyclodextrin (b-CD) and hydroxylpropyl-b-CD (HP-b-CD) were selected as chiral selectors. The results indicate that the separation window is greatly enlarged by b-CD concentration and that the separability and selectivity of phenothiazines are remarkably influenced by the concentrations of both b-CD and TTAB, as well as buffer pH. The interaction of thioridazine with b-CDs is considerably reduced in the presence of TTAB micelles due to competitive complexation of thioridazine with TTAB micelles, which is pH-dependent. As a result, effective enantioseparation of thioridazine is simultaneously achievable with that of trimeprazine and promethazine or ethopropazine in MEKC with addition of either b-CD or HP-b-CD, respectively, to a micellar citrate buffer containing TTAB at pH 3.5. Better enantioresolution of thioridazine in MEKC than in capillary zone electrophoresis can be obtained. application/pdf302777 bytesapplication/pdfzh-TWearbacteriologymethicillin resistanceStaphylococcus aureusjournal articlehttp://ntur.lib.ntu.edu.tw/bitstream/246246/2006111501233146/1/8183.pdf