Cheng K.-C.Lin J.-T.Liu W.-H.2019-07-112019-07-11201113309862https://scholars.lib.ntu.edu.tw/handle/123456789/413462In this study, ethanol extracts from 2-day fermented black soybean milk (FBE) by immobilized Rhizopus oligosporus NTU5 have been evaluated for both antioxidant and cytotoxic activities. The results reveal that a 2-day FBE had strong 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging effect (76 %). The extracts were further fractionated by silica gel column chromatography and an unknown compound, FBE5-A, was obtained, which exhibited strong antioxidant activity. IC50 of the DPPH scavenging effect of FBE5-A was 7.5 £gg/mL, which is stronger than a commonly used antioxidant, vitamin E (£\-tocopherol; 17.4 £gg/mL), and similar to vitamin C (ascorbic acid; 7.6 £gg/mL). The cytotoxic test demonstrated that extracts of 2-day fermented broth exhibited selective cytotoxic activity towards human carcinoma cells, Hep 3B (IC50=150.2 £gg/mL), and did not affect normal human lung fibroblasts, MRC-5 (p<0.05). The results indicate the potential applications of fermented black soybean milk as functional food, pharmaceutical or cancer therapy formula.Antioxidant activityBlack soybeanCytotoxic activityIsoflavoneRhizopus oligosporous[SDGs]SDG3Glycine max; Rhizopus; Rhizopus microsporus var. oligosporusjournal article2-s2.0-79953188759https://www.scopus.com/inward/record.uri?eid=2-s2.0-79953188759&partnerID=40&md5=e4477c07760dd0ee7bb7eb555683e734