Dutta, AvijitAvijitDuttaSHI-CHUEN MIAWChen, Tse-ChingTse-ChingChenChang, Chia-ShiangChia-ShiangChangHuang, Yu-LinYu-LinHuangLin, Yung-ChangYung-ChangLinLin, Chun-YenChun-YenLinHuang, Ching-TaiChing-TaiHuang2026-01-072026-01-072025-10-0800192805https://scholars.lib.ntu.edu.tw/handle/123456789/735135LAG-3 regulatory T cells suppress the effector but not the proliferative response of naïve cognate antigen-specific CD4 T cells in vivo. The Th1, Th2, and Th17 machineries in the suppressed CD4 T cells are impaired. Genomic study of the suppressed T cells revealed enhanced T cell receptor signalling with up-regulation of immune checkpoints, including PD-1 and PD-L1, and down-regulation of pro-inflammatory pathways. Although oxidative metabolism is reduced, the suppressed T cells retain proliferative capacity and acquire LAG-3 expression with proliferation. They acquire the capacity of LAG-3 regulatory T cells. They inhibit the IFN-γ response of co-cultured naïve CD4 T cells in vitro. Upon adoptive transfer, they rescue mice from lethal autoimmune pulmonitis in a dose-dependent manner. Our results implied a mechanism for the maintenance of long-lasting antigen-specific tolerance.entrueLAG‐3+ aTreg celleffector function‐suppressed proliferationhemagglutinin‐specific CD4+ T cellsinfectious tolerance[SDGs]SDG3journal article10.1111/imm.70046410603702-s2.0-105018478167