A.-H. LiaoS.-Y. WuH.-E. WangC.-H. WengM.-F. WuPAI-CHI LI2018-09-102018-09-102013-02http://scholars.lib.ntu.edu.tw/handle/123456789/381739Objective: In this study, albumin-shelled, targeted MBs (tMBs) were first demonstrated with the expectation of visualization of biodistribution of albumin-shelled tMBs. The actual biodistribution of albumin-shelled tMBs is of vital importance either for molecular imaging or for drug delivery. Motivation: Recently, albumin microbubbles (MBs) have been studied for drug and gene delivery in vitro and in vivo through cavitation. Targeted lipid-shelled MBs have been applied for ultrasound molecular imaging and conjugated with radiolabeled antibodies for whole-body biodistribution evaluations. The novelty of the work is that, in addition to the lipid tMBs, the albumin tMBs was also applied in biodistribution detection. Methods: Multimodality albumin-shelled, 18F-SFB-labeled VEGFR2 tMBs were synthesized, and their characteristics in mice bearing MDA-MB-231 human breast cancer were investigated with micro-positron-emission tomography (microPET) and high-frequency ultrasound (microUS). Results: Albumin-shelled MBs can be labeled with 18F-SFB directly and conjugated with antibodies for dual molecular imaging. The albumin-shelled tMBs show a lifetime in 30 min in the blood pool and a highly specific adherence to tumor vessels in mice bearing human breast cancer. Conclusions: From the evaluations of whole-body biodistribution, the potential of the dual molecular imaging probe for drug or gene delivery in animal experiments with albumin shelled MBs has been investigated. ? 2012 Elsevier B.V. All rights reserved.18F-SFB; Breast cancer; MicroPET; MicroUS; Targeted microbubbles[SDGs]SDG318F-SFB; Breast Cancer; MicroPET; MicroUS; Targeted microbubbles; Antibodies; Diseases; Drug delivery; Gene transfer; Molecular imaging; Positron emission tomography; Tumors; Ultrasonic applications; Mammals; albuminoid; contrast medium; fluorine; fluorocarbon; vasculotropin receptor 2; animal; article; Bagg albino mouse; echography; evaluation; experimental neoplasm; female; isotope labeling; metabolism; microbubble; mouse; nude mouse; positron emission tomography; scintiscanning; tumor cell culture; Albumins; Animals; Contrast Media; Female; Fluorine Radioisotopes; Fluorocarbons; Isotope Labeling; Mammary Neoplasms, Experimental; Mice; Mice, Inbred BALB C; Mice, Nude; Microbubbles; Positron-Emission Tomography; Tumor Cells, Cultured; Ultrasonography; Vascular Endothelial Growth Factor Receptor-2journal article10.1016/j.ultras.2012.06.0142-s2.0-84870249853WOS:000311488800003