2016-02-012024-05-18https://scholars.lib.ntu.edu.tw/handle/123456789/713606摘要：大蟬花(Cordyceps cicadae)為一種珍貴的傳統中藥材，民間主要用於抗疲勞、免疫調節及改善腎功能等。為了發展臺灣高經濟價值的藥用真菌產業，本計畫擬協助配合產商建立本土大蟬花子實體的優質量產技術平台，並進行一系列大蟬花子實體的活性多醣體物化特性與化學成分分析，以及體內免疫調節與其他生理活性和安全性評估。於第一年的工作項目，我們完成本土大蟬花菌株DNA序列之定序分析，並確認其基源的正確性及將其ITS序列登錄於NCBI的GenBank資料庫中(GenBank no. KP771879)。栽培試驗結果得知大蟬花菌絲的最佳生長溫&#64001;、pH值、碳氮源需求，及培養基組成。大蟬花在培養溫&#64001;24℃下，菌絲只需3-5天即佈滿太空包培養基，並在30-40天出菇。大蟬花子實體中含有3.42%水溶性粗多醣體(CP)和1.17%純化的多醣體(CPP)。CP和CPP分別含有6.29%及3.92%的蛋白質，其單糖組合主要為甘露糖，其次為葡萄糖和半乳糖。在分子量上，CP具有兩個主要的分子量群(21.5 kDa和3.1 kDa)和一個小分子量群(678 kDa)，而CPP則只有一個分子量群(24.4 kDa)且擁有三股螺旋構象。第二年的計畫將繼續改善大蟬花出菇的生產條件，以建立優質化子實體量產技術平台；此外，除了預計完成分析大蟬花多醣體的體內(in vivo study)免疫調節活性之外，也將評估大蟬花萃取物與其他成分的生理活性。本研究成果可作為生產高品質大蟬花子實體及其機能性產品開發之科學依據。<br> Abstract: Cordyceps cicadae is a precious ingredient of traditional Chinese medicine. It is popularly used to relieve fatigue, modulate immune system, improve renal function and others. In order to develop the high economic value medicinal fungi industry in Taiwan, this study intends to assist the collaborative company in developing an optimized platform technology for mass production of C. cicadae fruiting bodies, and conducting a series of investigations on the chemicals and physicochemical properties of its bioactive polysaccharides, as well as evaluating its in-vivo immunomodulatory and other physiological activities, and safety. In the first year of the project, we completed the local C. cicadae DNA sequence analysis and confirmed its authenticity, as well as submitting its ITS sequence to NCBI for recording in the GenBank database (GenBank no. KP771879). Culture experiments have resulted in knowing the best temperature, pH, carbon and nitrogen source requirements, and the medium composition for optimal growth of C. cicadae mycelia. At culture temperature 24℃, the C. cicadae mycelium took only 3-5 days to completely cover the medium in the space bag and fruiting in 30-40 days. The yields of crude polysaccharides (CP) and purified polysaccharides (CPP) of C. cicadae fruiting bodies were 3.42 % and 1.17 %, respectively. CP and CPP contained 6.29 % and 3.92 % of protein, repsetively. The predominant monosaccharide of CP and CPP was mannose, followed by glucose and galactose. There were apparent differences in molecular weight distribution between CP and CPP; two major (21.5 kDa and 3.1 kDa) and one minor (678 kDa) macromolecular populations were found in CP, whereas CPP contained only one macromolecular population (24.4 kDa) that possessed a triple helix conformation. The second year of the project will continue to improve the production conditions of C. cicadae fruiting bodies vis-&agrave;-vis establishing the platform technology for mass production of high quality C. cicadae fruiting bodies. In addition, besides completing the expected research on in vivo immunomodulatory activity of C. cicadae polysaccharides, other physiological activities of C. cicadae extracts and compounds will also be assessed. The results of this study can be used as a scientific basis for producing high-quality C. cicadae fruiting bodies and for their functional product development.大蟬花固態培養多醣體免疫調節安全性評估Cordyceps cicadaesolid state culturepolysaccharidesimmunomodulationsafety assessment