何子昌2006-07-262018-07-122006-07-262018-07-122002http://ntur.lib.ntu.edu.tw//handle/246246/26726High myopic maculopathy are one of the major causes of blindness. In the past several years retinal pigment epithelium transplantation has become the major direction of researches in the treatment of macular degeneration. Our previous studies demonstrated that photoreceptor-retinal pigment epithelium-choriocapillaris complex became ruptured in the elastic membrane of Bruch’ s membrane in form-deprivation myopic chicks. The staining of extracellular matrix has also decreased. This is similar to the lacquer crack formation in Bruch’s membrane in high myopia in human. These are the pathologic changes found in the substrates attached by retinal pigment epithelium. Accordingly, the successfulness of retinal pigment epithelium depends on the remodeling of the substrates. The gelatin-matrix transfer technique we developed is able to transfer extracellular matrix with or without cells. Our study demonstrates that immunolabeling of the exoplants after enzymatic treatments showing decreased finonectin, laminin, vitronectin and collagen IV staining.Transferred extracellular matrix was able to increase the efficiency of retinal pigment epithelial cells to substrates although they were previously treated by trypsin, dispase or collagenase. Our results suggest that the modification of substrates can be achieved by gelatin matrix technique transfer of extracellular matrix. In addition we found that DBA/2J mouse developed myopic changes with myopic fundus tessellation. In the present study, the retinal pigment epithelium and photoreceptors developed apoptosis, and combined RPE-extracellular matrix trasplantation significantly protected photoreceptor cell from apoptotic cell death in DBA/2J mouse and form-deprivation myopia chicks.application/pdf28610 bytesapplication/pdfzh-TW國立臺灣大學醫學院眼科Retinal pigment epitheliumgelatin matrix techniqueextracellular matrixretinal pigment epithelium transplantationapoptosisjournal articlehttp://ntur.lib.ntu.edu.tw/bitstream/246246/26726/1/902314B002187.pdf