2012-08-012024-05-14https://scholars.lib.ntu.edu.tw/handle/123456789/656510摘要：生醫材料發展多年，迄今仍然沒有理想之活髓治療及穿孔修復的材料。隨著組織再生的觀念應用於牙髓病治療，具生物活性之材料如鈣矽陶瓷，因具有誘導鈣化組織再生的能力，在活髓治療及穿孔修復上具有相當的潛力。γ-PGA高分子生物膠因其優異的生物相容性及材料性質可調整性高，被廣泛應用於傷口敷料及藥物釋放載體等。經前導實驗初步發現，我們研究團隊研發之鈣矽陶瓷結合高分子生物膠，具有提升牙髓及骨再生之效力。本計畫目的在結合這兩類材料，發展高分子鈣矽複合材料應用於活髓治療及穿孔修復，並藉由研發材料過程探索牙髓及骨再生之機轉。計畫分三年：第一年為以高分子鈣矽複合材料研發三種劑型，進行材料性質及細胞毒性分析。透過鈣矽成分比例與高分子分子量與官能基的調整，以應用於牙髓再生及穿孔修補。第二年研究高分子鈣矽複合材料對細胞行為之影響。由基因表現分析細胞表型的改變及發炎因子釋放，瞭解高分子鈣矽複合材料與細胞之間的相互作用，並探索細胞修復過程中生醫材料所創造有利於組織修復微環境的必要條件。第三年以動物實驗進一步評估所研發材料的安全性及有效性。未來更可將本計畫所研發的材料系統及研究架構應用於其他組織修復機制的探討與生醫材料的改良。<br> Abstract: The concept of tissue regeneration has become a trend in the pulp treatment. After years of research and development of the biomaterials, not an ideal material for vital pulp therapy and perforation repair is found. The ultimate goal of the regenerative pulp treatment is to reconstitute normal tissue continuum at the pulp-dentin border and root-bone interface. Bioactive ceramics have been used to repair bone defects, owing to their biological affinity to living bone. Its biocompatibility and the ability to induce the regeneration of calcified tissue have been proved to be very good. With its excellent biocompatibility and highly adjustability, γ-PGA has been applied in medicine as bio-adhesive and drug carrier.Our pilot study demonstrates that the polymer-calcium silicate composites had great potential to induce pulp and bone regeneration. The goal of this 3-year project is to develop the application of polymer-calcium silicate composites in the dental pulp regeneration/perforation repair, as well as to explore the associated mechanisms. In the first year, we will develop three different types of polymer-calcium silicate composites for pulp regeneration and perforation repair. We will analyze the physical and chemical properties of these novel composites and evaluate their cytotoxicity with MTT/LDH assay as well as Agar overlay assay.In the Second year, we will study the inductive effect and the interaction between the composites and cells. Through the cell mineralization staining and gene expression, we will analyze the evolution of the cell phynotype and the release of inflammatory factors. ALP, collagen Type I, DSPP, TGF, IL-1, IL-6, TNF, IL-8 will also be assessed by PCR to understand the best micro-environment for the tissue restoration that can be created by these biomedical materials. In the last year, we will evaluate the interrelationship of composites and pulp cells through animal experiments. The Animal studies are designed to evaluate the efficacy and biocompatibility of these composites. This project is aimed to demonstrate a complete process from understanding the mechanisms by underlying pulp-dentin and bone regeneration and testing their efficacy in vivo animal study to the manufacturing of the improved materials. It is also our hope to use the novel materials for the regeneration of other tissues in the future. Our systemic experimental designs will provide complete and detailed evaluation of the properties and bioactivity of the new materials. It will be a milestone for the development of biomaterials高分子鈣矽複合材料活髓治療牙髓再生穿孔修復γ-PGA生物活性生物相容性polymer-calcium silicate compositesvital pulp therapypulp regenerationperforation repair?-PGAbioactivitybiocompatibility.