2023-08-012024-05-13https://scholars.lib.ntu.edu.tw/handle/123456789/653318Lipid metabolism is important for cellular and organismal physiology[1-5]. However, little is known about how lipid metabolism is regulated by diets, largely due to the heterogeneity and complexity of diets and hosts. In addition, most dietary studies are limited to the standard food or starvation conditions [6]. Thus, how organisms may respond distinctively to different dietary composition remain largely elusive. Here, we take advantage of the genetically tractable model organism Caenorhabditis elegans and two of its bacterial diets, the standard E. coli OP50 (OP) and C. aquatica DA1877 (DA), to study how bacterial diets impact lipid metabolic program and homeostasis. We have identified a diet-dependent signaling pathway that involves two tissues, hypodermis and intestine, and two lipid types, phosphatidylcholine and sphingolipids, to regulate lipid droplet dynamics and triacyl glycerol levels. We propose to identify and characterize the metabolites, secretory proteins and receptors responsible for this novel cross-tissue signaling in regulation of lipid metabolism.秀麗隱桿線蟲;酸性的神經磷脂酶;硬脂醯-輔酶A 9-去飽和酶;S-腺苷甲硫氨酸合成酶;磷脂醯膽鹼;C. elegans; acid sphingomyelinase; S-adenosylmethionine synthase; lipid metabolism; PC