2019-03-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/684557摘要：為使農業永續發展，田間生態的多樣性漸受重視，已有許多研究投入動物植物的族群豐度及多樣性調查，但對於微生物的多樣性了解相較有限。作物內共生菌族群組成及多樣性影響作物生長發育及逆境的抗性，然而田間耕作模式是否會影響其組成目前所知甚少，而不同生育時期內共生菌群的變化也尚未深入探究，因此本計畫將以苗栗苑裡鎮有機及慣行水稻田為樣區，調查不同耕作方式下水稻秧苗及分蘗旺盛期根部內共生細菌族群組成及多樣性之差異，以了解內生菌對耕作方式的敏感度，以及不同生育時期的生理特性影響作物與內共生菌交互作用的情形，該調查結果可作為未來鑑定耕作方式之指標菌種及有益菌叢之參考。<br> Abstract: Because of the development of sustainable agriculture, the biodiversity in the field has drown more attention. Many reports are focused on the investigation of the richness and diversity of animals and plants in the fields, but there are less studies about the diversity of microbes. The composition and diversity of endophytes affect crop growth and development, and the stress resistance. However, we know little about the effect of farming practices on the endophyte diversity and the difference of endophyte diversity at different growth stages. This purpose of project is to investigate the effect of farming practices and different growth stages on the richness and diversity of rice root endophytes. We will collect the roots of rice seedlings and the plants with maximum tiller numbers from organic and conventional rice farms in Yuanli town at Miaoli County for endophyte population analysis. The results will show the sensitivity of endophytes to the farming practices and growth condition and provides resources for identification of bioindicators and beneficial microbes in the future.