2016-06-302024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/690013Deep caries, crown fracture, tooth preparation and abrasion may cause dentinal tubules exposed to the oral environment and make teeth susceptible to mechanical or thermal stimulation, causing inflammation and further damage to the pulp tissue.Over a long period, dentists havetreatedthe inflamed pulp tissue by removing the pulp tissue,cleaning and shaping the root canal system followed by canal obturation. In recent years, the researchers are paying more attention to maintain the vital pulp tissueas well as vital pulp therapy (VPT). The purpose of this study is to develop a novel strategies and formulations for effective drug delivery in the treatment of exposed dentinal tubules related diseases. This project has been arranged for three years, and the specific aims are as follows: First year: (1) To investigate the permeability of different anti-bacterial and anti-inflammation drugs by using the transwell dentin disc model (2) To investigate thepermeability and crystalization of the previously developed Ca mesoporous silicate/phosphate with different anti-bacterial and anti-inflammation drugs. Second year: (1) To establish an in vitro model using dental pulp cells to evaluate variable bioactivities of novel formulations. (2) To investigate the anti-bacterial and anti-inflamamtion ability and cytotoxity via thein vitro model. Third year: (1) To evaluate the anti-inflammatory and anti-bacterial effects of the novel formulations on dentin-pulp complex inan animal model. (2) To investigate the crystalization and occlusion performance of the novel trategies and preparations in the exposed dentinal tubules.