Tsai, C.-Y.C.-Y.TsaiChang, T.-L.T.-L.ChangUppala, R.R.UppalaChen, C.-C.C.-C.ChenFu-Hsiang, K.O.K.O.Fu-HsiangChen, P.-H.P.-H.ChenPING-HEI CHEN2018-09-102018-09-102005http://www.scopus.com/inward/record.url?eid=2-s2.0-31844448707&partnerID=MN8TOARShttp://scholars.lib.ntu.edu.tw/handle/123456789/314907A method of electrically detecting of protein described is developed using self-assembled multilayer gold nanoparticles (AuNPs) on a SiO2/Si substrate between gold electrodes. Electrical measurements are performed at room temperature using a probe station. A monoclonal antibody is immobilized on the top surface of the first layer of AuNPs (14nm). The second layer of AuNPs is formed through specific binding among a target antigen [hepatitis C virus, (HCV)], the monoclonal antibody, and the conjugate of a AuNP-polyclonal antibody. Once the specific binding among the monoclonal antibody, target antigen, and polyclonal antibody occurs, a significant electric current is detected through multilayer self-assembled gold nanoparticles between nanogap electrodes. No significant current (<1 pA) can be measured through a monolayer of AuNPs. A significant difference between the IV curves of the monolayer and the multilayer of AuNPs is used to identify whether the target antigen exists in the tested sample. ? 2005 The Japan Society of Applied Physics.Electrical detection; Gold nanoparticle; Multilayer; Protein chip; Self-assembled monolayer[SDGs]SDG3Gold; Monolayers; Nanostructured materials; Self assembly; Substrates; Electrical detection; Gold nanoparticles; Protein chip; Proteinsjournal article10.1143/JJAP.44.5711