蔡向榮2006-07-262018-07-092006-07-262018-07-091998http://ntur.lib.ntu.edu.tw//handle/246246/28665本計劃中擬從本地分離株中挑選具高 抗原性、高病原性之鴨瘟屢氏桿菌，利用 核酸增幅、基因選殖、基因互補、及同源 性基因交換等技術，來建構充分減毒但仍 具足夠抗原性的aroA 基因變異株。然而， 經過數十次接合反應及十多次轉型作用並 未獲得預期中的產物。 出血敗血性巴氏桿菌及鴨瘟屢氏桿菌 感染症，也就是所謂的家禽霍亂及水禽傳 染性漿膜炎，在水禽養殖業一直是一個嚴 重的問題。現在已有疫苗來控制這兩個疾 病，但是效果並不是非常好。大部分的菌 苗含有一種或數種血清型的細菌，經福馬 林不活化製成，通常只具部分保護效力; 且菌苗曾經引起暫時性的內毒素性休克。 目前以抑制數種自發性基因（ aroA）來製 成活毒減毒菌苗的方法已被採用。Pasteurella multocida (PM) and Riemerella anatipestifer (RA) infection, commonly known as fowl cholera and infectious serositis in waterfowl, continue to be a significant problem. Vaccines against both diseases are available but their efficacy has been poor. Most bacterins containing one or several serotypes of formalininactivated bacteria have induced protection. The bacterins also have caused transient endotoxin shock. The approach of attenuation based on several auxotrophic genes, such as aroA, has been employed to make live attenuated bacterins. The aroA gene of PM & RA will be cloned by complementation of the E. coli mutant after electroporation with a genomic library constructed in plasmid. Their sequences will be determined by Sanger‘s dideoxy method. The genes will be inactivated by insertion of a antibiotic resistance gene and reintroduced by allelic exchange into the chromosome of the parental PM & RA. The mutants’ virulence will be examined in a mouse model.application/pdf49272 bytesapplication/pdfzh-TW國立臺灣大學獸醫學系暨研究所aroA 突變株出血敗血性巴氏桿菌鴨瘟屢氏桿菌aroA mutantPasteurella multocidaRiemerella anatipestifer[SDGs]SDG3reporthttp://ntur.lib.ntu.edu.tw/bitstream/246246/28665/1/872313B002088.pdf