2023-01-012024-05-15https://scholars.lib.ntu.edu.tw/handle/123456789/662212染色體粒端有一種非編碼RNA的生成，這種RNA稱為TERRA，它包含有染色體粒端重複的序列，在人類的癌細胞當中，TERRA會與染色體粒端的DNA結合，形成RNA-DNA雜交的結構，這個RNA-DNA雜交的結構在酵母菌中會影響DNA的複製與增加染色體互換的效應，而導致基因體的不穩定。此計劃要研究TERRA是否在人類癌細胞中影響染色體粒端DNA的互換，以及探討如何影響的機制，期望能進步發展出針對抑制癌細胞生長的方法。 Telomeric ends actively synthesize a heterogeneous population of long noncoding RNAs, which contain telomeric repeats, named “TERRA”. TERRA forms RNA–DNA hybrid structures at telomeres in human cancer cells. The unresolved TERRA:telomere hybrids (telomeric hybrids) can induce genome instability by impairing DNA replication and promoting recombination in budding yeast. This subproject is to study whether TERRA is involved in recombination for alternative lengthening of telomeres (Aim1) and how it participates the recombination events in ALT cancer cells (Aim2). The long-term goal is to target ALT pathway and develop a therapeutic method for cancer therapy (Aim3).染色體粒端 非編碼RNA RNA-DNA雜交;Telomere; TERRA; R-loop