Han, H.-W.H.-W.HanHsu, S.-H.S.-H.HsuSHAN-HUI HSU2020-03-022020-03-022017https://scholars.lib.ntu.edu.tw/handle/123456789/467568Chitosan has been considered as candidate biomaterials for neural applications. The effective treatment of neurodegeneration or injury to the central nervous system (CNS) is still in lack nowadays. Adult neural stem cells (NSCs) represents a promising cell source to treat the CNS diseases but they are limited in number. Here, we developed the core-shell spheroids of NSCs (shell) and mesenchymal stem cells (MSCs, core) by co-culturing cells on the chitosan surface. The NSCs in chitosan derived co-spheroids displayed a higher survival rate than those in NSC homo-spheroids. The direct interaction of NSCs with MSCs in the co-spheroids increased the Notch activity and differentiation tendency of NSCs. Meanwhile, the differentiation potential of MSCs in chitosan derived co-spheroids was significantly enhanced toward neural lineages. Furthermore, NSC homo-spheroids and NSC/MSC co-spheroids derived on chitosan were evaluated for their in vivo efficacy by the embryonic and adult zebrafish brain injury models. The locomotion activity of zebrafish receiving chitosan derived NSC homo-spheroids or NSC/MSC co-spheroids was partially rescued in both models. Meanwhile, the higher survival rate was observed in the group of adult zebrafish implanted with chitosan derived NSC/MSC co-spheroids as compared to NSC homo-spheroids. These evidences indicate that chitosan may provide an extracellular matrix-like environment to drive the interaction and the morphological assembly between NSCs and MSCs and promote their neural differentiation capacities, which can be used for neural regeneration. ? 2017 Elsevier B.V.Cell interaction; Cell transplantation; Central nervous system injury; Chitosan; Neural stem cells[SDGs]SDG3Chitosan; Enzyme inhibition; Flowcharting; Neurophysiology; Stem cells; Tissue; Cell interaction; Cell transplantation; Central nervous system injuries; Central nervous systems; Extracellular matrices; Mesenchymal stem cell; Neural differentiations; Neural stem cell; Cell culture; chitosan; Notch receptor; biomaterial; chitosan; animal cell; Article; cell differentiation; cell interaction; cell lineage; cell survival; coculture; controlled study; extracellular matrix; in vivo study; locomotion; mesenchymal stem cell; mouse; nerve regeneration; neural stem cell; nonhuman; priority journal; survival rate; zebra fish; animal; central nervous system; chemistry; cytology; drug effect; human; mesenchymal stroma cell; multicellular spheroid; neural stem cell; signal transduction; Animals; Biocompatible Materials; Cell Differentiation; Central Nervous System; Chitosan; Coculture Techniques; Humans; Mesenchymal Stromal Cells; Neural Stem Cells; Signal Transduction; Spheroids, Cellular; Zebrafishjournal article10.1016/j.colsurfb.2017.07.0362-s2.0-85025176576https://www.scopus.com/inward/record.uri?eid=2-s2.0-85025176576&doi=10.1016%2fj.colsurfb.2017.07.036&partnerID=40&md5=cab0a3f0bf63695d70be6d45994903b1