Shih, Hsiu ChenHsiu ChenShihLee, Tse AngTse AngLeeHSIAO-MEI WUKo, Ping LiangPing LiangKoTung, Yi ChungYi ChungTung2022-03-172022-03-172018-01-019781510897571https://scholars.lib.ntu.edu.tw/handle/123456789/597751This paper studies the effects of hypoxia-inducible factor (HIF) inhibitor on endothelial cell migration under oxygen gradients. The experiments are conducted using microfluidic cell culture devices capable of generating oxygen gradients. The oxygen gradients are generated using a spatially confined chemical reaction method to eliminate direct chemical addition into cell culture medium, and the device is compatible with conventional cell incubators. The results indicate that the HIF inhibitor has minimal effects on endothelial polarized cell migration toward low oxygen side. The developed platform provides a powerful tool to study the endothelial cell behaviors under various oxygen conditions and drugs.Endothelial cells (ECs) | Human umbilical vain endothelial cells (HUVECs) | Oxygen gradients | YC-1conference paper2-s2.0-85079740161https://api.elsevier.com/content/abstract/scopus_id/85079740161