2021-01-012024-05-13https://scholars.lib.ntu.edu.tw/handle/123456789/653864在精子減數分裂中，染色體複製一次但接連分離兩次，產生單倍體。這個過程是在同一個細胞周期M時期作用，但細胞如何在第一次減數分裂完成後進入第二次減數分裂仍未知。本實驗室在線蟲精原細胞進行活體觀察，發現抑制蛋白酶體和抑制轉譯都會使精原細胞停留在第一次減數分裂，顯示在兩次分裂中間有重要的分子降解及合成。本計畫旨在探討究竟第一次到第二次分裂是如何調控，我們將結合活體攝影、小分子抑制劑以及RNA定序的方式來尋找可能在這過程中扮演角色的分子。 In male meiosis, the chromosomes undergo two consecutive separation events after one round of duplication. Because the two division events are within the same M phase, it is unclear how the cells transit to second division upon completion of first division. Using C. elegans as a model, we observed the progression of male meiotic divisions through time-lapse recording. We found that inhibition of either proteasome or translation would cause stall of male meiosis in first division, indicating relevant proteins need to be generated or degraded during the transition. In this proposal, we aim to explore how the meiosis I to meiosis II transition is regulated. We will adapt both candidate and genomic approaches to identify key regulators精子減數分裂細胞周期紡綞體檢查點蛋白&#37238體轉譯male meiotic divisioncell cyclespindle assembly checkpointproteasometranslation