2012-08-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/682828摘要：肢體組織在發育的過程之中，其細胞構成由本來的軟骨組織經過內皮細胞發育的血管組織後，將進一步鈣化形成骨骼組織，同時在關節處發育成韌帶組織後構成一複雜的組織系統，其中內皮細胞的遷移決定了骨骼組織的形成。因此，研究內皮細胞在不同影響因子下的遷移行為即成為一個重要的課題。 另外一方面，現今利用微系統晶片進行細胞遷移的研究，主要是以有關基材硬度牽引力、流體剪力及生長因子等因素，如何影響細胞運動的研究最多，其中，相關文獻利用微流道晶片進行各種環境因子的刺激，期望可以了解細胞遷移之機制與影響的程度。然而，受限於微流道晶片技術的限制，相關微流道晶片在進行各種環境因子的刺激時，鮮少針對光刺激的部份進行研究。因此，本紫計劃將發展細胞晶片，利用此一細胞研究微系統晶片平台，將可以仔細地針對相關光刺激細胞遷移的現象進行研究。 <br> Abstract: The cell migration is one of the most important phenomenons in cell biology. It directly affects wound healing, tissue regeneration, and tumor cell metastasis. As a consequence, it is intriguing to employ nanotechnologies to study this cell behavior. Utilizing nanotechnologies, not only chemical/mechanical cue can be studied but also photo-induced mechanism can be explored. This will not only offer the fundamental understanding of tissue regeneration but also give us the insight of cancer cell development. To achieve above ambitious goals, we will develop opto-electronic biochip as the cell analysis platform. Traditionally, the cell migration study is accomplished in cell culture dishes. Recently, the micro-environmental effect has also been studied in microfluidic channel based on the advancement of micro-fabrications. However, few of studies explore the photo-induced cell migration and non-contact cue of cell migration. Because of the limitation measurement tools, in addition, it is rarely to study these factors in traditional cell migration assays. To overcome these obstacles, we will fabricate the cell chip to study the photo-induced cell migration phenomena of cancer cells. This will integrate the traditional understandings of cell biology and innovative applications of infra-red nanotechnologies. Moreover, the developed cell chip can be stimulated and monitored by both optical and electrical methods under well-controlled microenvironments. Utilizing the developed cell monitoring platform, the tumor cell will be used as the cell model to study the cell migration phenomena affected by chemical, mechanical, and photonic cues. The result of this study can be used to lay down the effects of different cell migration factors.細胞晶片微流道晶片cell chipmicrofluidic chip