Huang, Chih-ChingChih-ChingHuangChiu, Sheng-HsienSheng-HsienChiuHuang, Yu-FenYu-FenHuangChang, Huan-TsungHuan-TsungChang2009-09-102018-07-102009-09-102018-07-102007http://ntur.lib.ntu.edu.tw//handle/246246/165256http://ntur.lib.ntu.edu.tw/bitstream/246246/165256/1/78.pdfAn aptamer modified gold nanoparticles (Apt-AuNPs) based molecular light switching sensor has been demonstrated for the analysis of breast cancer markers (platelet-derived growth factors (PDGFs) and their receptors) in homogeneous solutions. The PDGF binding aptamer has a unique structure with triple-helix conformation that allows N,N-dimethyl-2,7-diazapyrenium dication (DMDAP) and PDGF bindings. The fluorescence of DMDAP is almost completely quenched by Apt-AuNPs when it intercalates with the aptamers. Owing to high magnitudes of increases (up to 40-fold) in the turn-on fluorescence signals of DMDAP/Apt-AuNP upon PDGFs binding, the approach is highly sensitive for the detection of PDGFs. The DMDAP/Apt-AuNP probe specifically and sensitively detected PDGFs under optimal concentrations of salts and DMDAP. We also demonstrated that the Apt-AuNPs are effective selectors for enrichment of PDGF-AA from large-volume samples. The approach allows detection of PDGF-AA at a concentration down to 8 pM, showing better sensitivity than other signal aptamers. By conducting a competitive assay, we demonstrated the determination of PDGF receptor-α with LOD of 0.25 nM when using the DMDAP/Apt-AuNP as a probe. ? 2007 American Chemical Society.application/pdf253816 bytesapplication/pdfen-US[SDGs]SDG3Aptamers; Platelet derived growth factors (PDGF); Chemical sensors; Concentration (process); Fluorescence; Gold; Nucleic acids; Salts; Solutions; Nanoparticles; aptamer; n,n dimethyl 2,7 diazapyrenium dication; nanoparticle; platelet derived growth factor; platelet derived growth factor receptor; tumor marker; unclassified drug; article; breast cancer; fluorescence resonance energy transfer; molecular probe; nanosensor; protein analysis; Aptamers, Nucleotide; Aza Compounds; Base Sequence; Binding, Competitive; Biosensing Techniques; Breast Neoplasms; Gold; Humans; Light; Nanoparticles; Platelet-Derived Growth Factor; Pyridinium Compounds; Sensitivity and Specificity; Spectrometry, Fluorescence; Tumor Cells, Culturedjournal article10.1021/ac07070752-s2.0-34447326732WOS:000247611600004http://ntur.lib.ntu.edu.tw/bitstream/246246/165256/1/78.pdf