Wu A.M.JIA-HAU LIU2022-04-252022-04-2520190282-0080https://www.scopus.com/inward/record.uri?eid=2-s2.0-85064693093&doi=10.1007%2fs10719-019-09865-3&partnerID=40&md5=7db77e04847c062288e96707acfd2c19https://scholars.lib.ntu.edu.tw/handle/123456789/607807Lectins, in combination with our established enzyme-linked lectin sorbent assay (ELLSA) and inhibition study, have been used as powerful tools in many glycoconjugate recognition studies. In this short review, we highlight the following: (i) The recognition profiles of Gal/GalNAc-specific lectins were updated and upgraded. (ii) Based on the cross-specificities of applied lectins, a new classification system was introduced. (iii) Applications of lectins for the detection and identification of N-glycan and/or Tn glycotope in glycoconjugates were intergraded. (iv) The polyvalency of the glycotopes in glycans was found to play a critical role in glycan–lectin recognition. This is an unexplored area of glycobiology and one of the most promising directions toward the coming glycoscience transformation.[SDGs]SDG3review10.1007/s10719-019-09865-3309935182-s2.0-85064693093