臺灣大學: 動物科學技術學研究所吳信志馬耘逸Ma, Yun-YiYun-YiMa2013-03-272018-06-292013-03-272018-06-292010http://ntur.lib.ntu.edu.tw//handle/246246/2538573 摘要 本研究主旨在探討小鼠原核胚經雙基因共同注射後，於體外培養至二細 胞時期再進行胚移置對產製攜帶雙基因轉殖小鼠效率之影響。轉殖基因之構築 係以乳腺專一表現之牛74 英文摘要 Generation of Transgenic Mice Harboring Human Pulmonary Surfactant-related Protein B/C Genes Yun-Yi Ma Abstract The purpose of this study was to investigate the effect of advanced culture to two-cell stage prior to co-injected mouse eggs on pups born and transgene integration efficiency by pronuclear gene microinjection. Transgenes were constructed by using a mature surfactant protein B (mSP-B) and C (mSP-C) cDNA fragment equipped with bovine4287112 bytesapplication/pdfen-US雙基因共同注射基因轉殖小鼠B 型肺臟表面張力蛋白質C型蛋白質乳汁microinjectiontransgenic micesurfactant protein Bsurfactant protein Cmilkthesishttp://ntur.lib.ntu.edu.tw/bitstream/246246/253857/1/ntu-99-R97626012-1.pdf