2021-01-012024-05-18https://scholars.lib.ntu.edu.tw/handle/123456789/704342番茄是世界重要蔬菜與水果，但其經濟生產常受尻腐病危害而造成損失。尻腐病是一種生理障礙，它可能是由於植物細胞鈣離子的異常分配或活性氧累積所造成的。然而，目前並沒有明確證據可得知以上兩種說法何者正確。低鈣與高鹽逆境皆可誘導番茄尻腐病發生，但其作用機制仍不清楚。本研究提出四種假說，並認為不同逆境誘導尻腐病的生理機制不同，而本研究將探討低鈣與高鹽逆境誘發尻腐病的生理機制。故本研究的內容涵蓋有(1)探討低鈣與高鹽處理對番茄尻腐病發生率的影響；(2)探討低鈣與高鹽處理對授粉後不同天數果實細胞層級鈣離子濃度、ROS與抗氧化防禦系統之影響。透過上述研究將幫助我們更佳瞭解尻腐病的細胞與生理調控機制，也將幫助我們找到防治尻腐病最適當的方式。 Tomato (Solanum lycopersicum L.) is an important vegetable and fruit in the world, but its economic production is often damaged by blossom-end rot (BER). BER is a physiological disorder, it may cause by aberrant partitioning of cellular calcium ion (Ca2+) or accumulation of reactive oxygen species (ROS) in plant. However, there is no clear evidence to know which of these two statements is correct. Both low calcium and high salinity stress can induce tomato BER, but its mechanism is also remains unclear. This study proposes four hypotheses and suggest that the physiological mechanisms of BER induced by different stresses are different. In this study, we will explore the physiological mechanisms of BER induced by low calcium and high salt stress. Accordingly, this study will include (1) Investigating the effects of low calcium and high salinity stress on BER incidence; (2) Analyzing the effects of low calcium and high salinity stress treatment on the concentration of Ca2+ at the cellular level, ROS and antioxidative defense system in fruits at different days after pollination (DAP). Above research will help us better understand the cellular and physiological regulatory mechanisms of BER, and also help us find the most appropriate way for BER prevention in the future.番茄尻腐病鈣活性氧高鹽逆境tomatoblossom-end rot (BER)calciumreactive oxygen species (ROS)high salinity stress