Anand, AnkurAnkurAnandSu, Feng-YiFeng-YiSuChen, Tse-HaoTse-HaoChenChen, Yung-FuYung-FuChenChen, Yit-TsongYit-TsongChen2025-07-182025-07-182025-06-17https://scholars.lib.ntu.edu.tw/handle/123456789/730738The measurement of thyroid hormones in serum is widely regarded as the most valuable single laboratory tool for assessing thyroid function. This study presents a highly sensitive tin disulfide nanosheet-fabricated field-effect transistor (SnS2-FET) designed for the detections of human thyroid-stimulating hormone (hTSH) and thyroxine (T4). By co-modifying an antibody (AbTSH for detecting hTSH), or a DNA aptamer (AptT4 for detecting T4), with polyethylene glycol (PEG) on the SnS2-FET channel surface, the PEG:AbTSH/SnS2-FET and PEG:AptT4/SnS2-FET devices achieve highly sensitive and selective detections of hTSH and T4, respectively, even in a high ionic strength buffer (1× PBS) or undiluted serum. With a low limit of detection (in the femtomolar level) and a wide linear working range (spanning at least 6 orders of magnitude of analyte concentration), the PEG:AbTSH/SnS2-FET immunosensor and PEG:AptT4/SnS2-FET aptasensor can detect the hTSH and T4 levels encountered in the spectrum of thyroid disorders. Notably, these specific receptor-modified SnS2-FET devices display negligible cross-reactivity with other pituitary hormones or serum components. This research indicates that the nanoelectronic SnS2-FET sensor platforms hold significant potential for point-of-care clinical diagnostics, particularly for the ultrasensitive detection and early screening of medical conditions.enantibodyaptamerfield-effect transistor biosensorpoint-of-care diagnosticspolyethylene glycolthyroid-stimulating hormonethyroxine[SDGs]SDG7journal article10.1021/acssensors.5c00115