Liao, Hsien-ChiHsien-ChiLiaoTsou, Kuan-ChuanKuan-ChuanTsouWAN-TING HUNGCHAO-WEN LUWu, Ying-SyuanYing-SyuanWuSu, Wei-ChingWei-ChingSuJu, Yu-TenYu-TenJuHSAO-HSUN HSUYoung, Tai-HorngTai-HorngYoungJIN-SHING CHEN2025-12-172025-12-172025-11https://scholars.lib.ntu.edu.tw/handle/123456789/734721This study aimed to examine the histological and mechanical effects of cryopreservation on human aortic tissues, focussing on storage duration and conditions. Assessments included smooth muscle cell integrity, elastic fibre preservation, and endothelial viability. Cryopreservation with dimethyl sulphoxide (DMSO) significantly reduced smooth muscle cell nuclei loss and maintained elastic fibre integrity. However, elastic fibre thickness increased after 12 months. Isolectin B4 staining showed reduced endothelial cell viability across all groups. No significant changes were observed in mucoid extracellular matrix accumulation or elastic fibre fragmentation. These findings suggest that cryopreservation with DMSO effectively maintains structural integrity for up to 12 months but requires refinement to address endothelial and biomechanical concerns. Cryopreserved aortic allografts demonstrated structural and functional performance when stored at low temperatures, confirming their viability for reconstructive surgeries. The study highlights the importance of the timely utilisation of cryopreserved grafts and optimising preservation techniques to advance surgical applications.entrueaortic homograftsbiomaterialcryopreserved aortatracheal regeneration[SDGs]SDG15journal article10.1177/20417314251397592413627932-s2.0-105024202056