廖中明2006-07-252018-06-292006-07-252018-06-292004http://ntur.lib.ntu.edu.tw//handle/246246/10768第二年研究工作針對砷於吳郭魚( Oreochomis mossambicus)各器官內之毒理動力及劑量反應關係進 行調查，並結合以AUC 為基礎之急性毒模式及藥理動 態模式以建立各器官之平衡砷濃度與死亡率之關係。7 天暴露實驗顯示，魚體器官組織中生物濃縮因子值最 高者為胃，最低為肌肉，其值分別為9.56 及1.04， 各器官生物濃縮因子大小排序如下：胃>腸>肝>鰓> 肉，顯示吳郭魚對砷具有累積能力。急性毒實驗顯示 24 及96 小時魚體半致死濃度分別為69.06 及28.86 mg L-1。以Hill 方程式建立之劑量反應關係顯示肌肉、鰓 與肝有較顯著之效應曲線，其半數致死時各器官砷濃 度分別為26.6、62.5 及78.5 µg g-1乾重。本研究建 議選取吳郭魚鰓之砷濃度作為風險評估之依據。The objective of our work in the second year is to determine the organ-specific toxicokinetics and dose-responses of arsenic (As) burdens in tilapia. We linked kinetically an AUC-based acute toxicity model and a pharmacodynamic model to derive dose-response relationships between equilibrium organ-specific As concentrations and mortality effects. The 7-d exposure test revealed that the highest bioconcentration factor (BCF) is found in the stomach and the lowest one is in the muscle, and the values are 9.56 and 1.04, respectively. The order of BCF was stomach > intestine > liver > gill > muscle. The 7-d acute toxicity bioassay showed that 24 and 96-h LC50 for tilapia exposed to As are 69.06 and 28.68 mg L-1, respectively. Dose-response relationships followed the refined Hill equation indicated that that the muscle, gill and liver have a relative steep sigmoid dose-response profile in that IEC50 are 26.6, 62.5 and 78.5 µg g-1 dry wt, respectively. We suggested that the gill could be used as a surrogate in site-specific risk assessment.application/pdf49278 bytesapplication/pdfzh-TW國立臺灣大學生物環境系統工程學系暨研究所砷吳郭魚急性毒生物累積烏腳病ArsenicTilapiaAcute toxicityBioaccumulationBlackfoot diseasereporthttp://ntur.lib.ntu.edu.tw/bitstream/246246/10768/1/922313B002031.pdf