Cheng C.-W.Yu J.-C.Wang H.-W.CHIUN-SHENG HUANGShieh J.-C.Fu Y.-P.Chang C.-W.Wu P.-E.Shen C.-Y.2020-03-232020-03-2320100009-8981https://www.scopus.com/inward/record.uri?eid=2-s2.0-72449192625&doi=10.1016%2fj.cca.2009.11.009&partnerID=40&md5=63002c9a4e8d944876b4114fa33e1e1chttps://scholars.lib.ntu.edu.tw/handle/123456789/477823Background: Tumor invasiveness and metastasis in cancer progression is manifested by epigenetic abnormality. However, it remains unknown whether transcription regulation of matrix metalloproteinase-11(MMP-11) and cytoskeleton-20 (CK-20) genes for the homoeostasis of epithelial/connective interface that can enhance cell dissemination and invasion may act as alternative mutators to tumor clinicopathology. Methods: Paired cancerous and tumor-adjacent normal tissues from 72 breast cancer patients were assayed for the expression of MMP-11 and CK-20 by using real-time RT-PCR. The expression profiles were evaluated for the association with clinicopathological factors. Results: Breast tumor tissues displayed higher expression levels of MMP-11 and CK-20 than those of the adjacent non-cancerous tissues. Overexpression of either MMP-11 or CK-20 correlated with patients having poorly differentiated tumors (PMMP-11 = 0.01 and PCK-20 = 0.05) and lymph node metastasis (LNM) (PMMP-11 = 0.004 and PCK-20 = 0.001). A synergistic effect between MMP-11 and CK-20 on risk elevation was significant in patients with advanced tumor stage (OR = 2.03, 95%CI = 1.10-3.77) and LNM (OR = 2.83, 95%CI = 1.20-4.71). Additionally, patients lacking progesterone receptor exhibited high expression of MMP-11 and CK-20. Conclusion: We demonstrate that MMP-11 and CK-20 are probable prognostic markers whose expression reflects the stages of tumor differentiation and LNM of breast cancer. ? 2009 Elsevier B.V. All rights reserved.[SDGs]SDG3cytokeratin 20; estrogen receptor; messenger RNA; progesterone receptor; stromelysin 3; adult; age; aged; article; breast cancer; cancer staging; cell spreading; clinical feature; controlled study; disease association; family history; female; gene expression; genetic epigenesis; homeostasis; human; human tissue; immunohistochemistry; immunoreactivity; lymph node metastasis; major clinical study; priority journal; real time polymerase chain reaction; risk factor; transcription regulation; tumor volume; Adult; Aged; Antibodies; Breast Neoplasms; Female; Gene Expression Regulation, Neoplastic; Genetic Predisposition to Disease; Humans; Immunohistochemistry; Keratin-20; Lymphatic Metastasis; Matrix Metalloproteinase 11; Middle Aged; Neoplasm Staging; Prognosis; Receptors, Estrogen; Receptors, Progesterone; RNA, Messenger; Tumor Markers, Biologicaljournal article10.1016/j.cca.2009.11.009199142292-s2.0-72449192625