林景堉陳慧祺游山林游秋綿李玫萱周雨青陳建仁2010-10-042018-06-292010-10-042018-06-292007http://ntur.lib.ntu.edu.tw//handle/246246/210496The aim of this study was to evaluate the sensitivity, specificity, reliability and reproducibility of the EasyChip? HPV blot for human papillomavirus (HPV) genotyping. Type-specific sensitivity and specificity for 39 types of HPV (HPV 6, 11, 16, 18, 26, 31, 32, 33, 35, 37, 39, 42, 43, 44, 45, 51, 52, 53, 54, 55, 56, 58, 59, 61, 62, 66, 67, 68, 69, 70, 72, 74, 82, CP8061, CP8304, L1AE5, MM4, MM7 and MM8) were examined. The operating environment, reliability, reproducibility and blot interpretation were assessed by a quality assurance system. Each batch experiment contained samples from 89 cervical specimens and 7 extrinsic controls. Caski, HeLa and Jurkat cells, male human blood cell DNA and sterile water were used to assess reliability. Furthermore, pairs of sibling controls were used to assess reproducibility. The overall sensitivity of HPV detection was 1-50 copies of HPV genome equivalent. There was no cross-reactivity with amplicons of other HPV genotypes. One hundred batch experiments demonstrated that the reliability was excellent. The intra-batch and inter-batch reproducibility was 98 and 97%, respectively. It was concluded that the EasyChip? HPV blot is a highly sensitive, reliable and reproducible tool for detection and identification of HPV genotypes. ? 2006 Elsevier B.V. All rights reserved.en-USHuman papillomavirus; Reliability; Reproducibility; Sensitivity; Specificity[SDGs]SDG3DNA; water; article; blood cell; blotting; cancer cell; Caski cell; controlled study; cross reaction; gene amplification; genotype; HeLa cell; human; human cell; leukemia cell line; priority journal; quality control; reliability; reproducibility; sensitivity and specificity; virus detection; virus gene; virus genome; virus identification; Wart virus; Human papillomavirusjournal article10.1016/j.jviromet.2006.10.004