Huang HLi KLee YMING-JU CHENYA-JANE LEE2022-04-252022-04-25202100218561https://www.scopus.com/inward/record.uri?eid=2-s2.0-85110258535&doi=10.1021%2facs.jafc.1c01547&partnerID=40&md5=5386876741f7f86e93a97658cb92f5f6https://scholars.lib.ntu.edu.tw/handle/123456789/605857Gut dysbiosis is a major contributor to adverse chronic kidney disease (CKD) progression, and microbiota-based strategies could be considered as a novel therapeutic and preventative target. In this study, a probiotic screening platform based on gut-derived uremic toxin-reducing probiotics was developed and the underlying mechanism was further verified through a 0.2% adenine-induced CKD mouse model. Two strains (Lactobacillus paracasei and Lactobacillus plantarum) were selected due to their high clearance ability and named Lactobacillus mix (Lm). The results showed that Lm significantly improved the kidney function by reducing kidney injury and fibrotic-related proteins. Furthermore, Lm decreased oxidative stress and proinflammatory reactions and elevated immune responses in the kidney. Importantly, Lm reversed gut dysbiosis and restored the abundance of commensal bacteria, especially short-chain fatty acid producers, leading to improved intestinal barrier integrity via modulation of microbial composition and metabolite production. Taken together, these findings provided evidence that Lm could be a preventive approach against CKD. ? 2021 American Chemical Society.chronic kidney diseasegut dysbiosisgut-derived uremic toxinsLactobacillusprobiotic screening platformBacilliDiagnosisMetabolitesVolatile fatty acidsChronic kidney diseaseImmune responseIntestinal barriersLactobacillus plantarumMetabolite productionMicrobial compositionProinflammatoryShort-chain fatty acidsProbioticsprobiotic agentanimalbacteriumchronic kidney failuredisease exacerbationdown regulationintestine floramouseAnimalsBacteriaDisease ProgressionDown-RegulationGastrointestinal MicrobiomeMiceRenal Insufficiency, Chronic[SDGs]SDG3journal article10.1021/acs.jafc.1c01547341706592-s2.0-85110258535