2023-01-012024-05-13https://scholars.lib.ntu.edu.tw/handle/123456789/654160本子計畫的研究目標為利用3D列印開發三維生物複合材料支架，主要的應用為神經幹細胞培養與分化。成年人的大腦神經細胞衰退是不可逆的且無法再生，相關的組織工程研究皆致力於尋找如何有效地在體外培養出神經細胞的方法以提供新型療程，因此本研究將會深入探討生物支架於神經幹細胞分化與神經元突的生長(第三子計畫主要內容)的重要性。在支架材料的選擇上，由於生物性複合材料已被證實除了可增加生物支架的結構穩定性，更可以促進生物活性以提供良好的細胞生長環境，本子計畫將會延續此研究趨勢，著重於複合材料的應用，並使用第一子計畫所開發的生物活性玻璃(Bioactive glass)探討其混合比例對於支架製作及腦神經幹細胞培養環境的影響。最後，本子計畫將會導入機器學習技術建立一模型預測兩大研究重點: 1) 不同複合材料比例與製程參數對於最終生物支架結構的外型與內部結構幾何的影響與 2) 不同材料性質與生物支架結構對於最終腦神經幹細胞的生長狀態，期望可提供該領域的研究團隊設計生物活性玻璃/膠體複合支架的指導方針。 This subproject (Subproject 2) aims to utilize 3D printing to develop three-dimensional composite porous bioscaffold with the target application on neural stem cell culture and differentiation. Recent research trend focuses on identifying an effective way to conduct neural stem cell culture in vitro to create novel treatment for non-reversible neurodegenerative disorder diseases. The goal of this project is to explore the importance of three-dimensional composite bioscaffold on neural stem cell culture and differentiation (the goal of Subproject 3). This subproject will use gel-based materials mixed by bioactive glass powders (results from Subproject 1) with different weight ratios to fabricate composite bioscaffolds with different material compositions, aiming to identify the optimal fabrication recipe to boost neural stem cell culture. Finally, this subproject will establish a machine learning model to predict: 1) effects of material compositions on bioscaffold geometrical accuracy and structural strength, and 2) effects of bioscaffold geometry and structure on neural stem cell culture performance. Findings will be summarized to create a design guideline for three-dimensional composite bioscaffold fabrication.生物支架;積層製造;3D列印;機器學習;Bioscaffold;Additive Manufacturing;3D Printing;Machine Learning