Lee S.-L., Chen Y.-F., Dong C.-Y.Chen Y.-F., Dong C.-Y.Lee S.-L.Chen, Y.-F.Y.-F.ChenCHEN-YUAN DONGYANG-FANG CHEN2021-07-282021-07-28201916057422https://www.scopus.com/inward/record.uri?eid=2-s2.0-85067828972&doi=10.1117%2f12.2507175&partnerID=40&md5=452e0f9252547fedbc6a915af11b800ehttps://scholars.lib.ntu.edu.tw/handle/123456789/573401Cornea is one of the collagen-rich connective tissues and plays an important role in vision. While X-ray scattering techniques are able to determine bulk structure of cornea, second harmonic generation microscopy can reveal depthdependent details of the corneal stroma comprised of a layered network of fibrillar collagen. In this work, we used Fast Fourier Transform second harmonic generation microscopy as a tool to determine the directionality of corneal stroma as a function of depth. Our results also display the position dependent difference of corneal stroma architecture. ? COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.Collagen; Fast Fourier transforms; Harmonic analysis; Microscopic examination; Musculoskeletal system; Network layers; Nonlinear optics; X ray scattering; Bulk structure; Connective tissues; Cornea; Depth dependents; Fibrillar collagens; Layered network; Position dependents; Second harmonic generation microscopies (SHG); Harmonic generationconference paper10.1117/12.25071752-s2.0-85067828972