Shen S.-T.Cheng Y.-S.Shen T.-Y.Yu J.Y.-L.2019-07-222019-07-22200600166480https://scholars.lib.ntu.edu.tw/handle/123456789/414375We have cloned FSH-£] cDNA from duck pituitary gland by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA end (RACE) methods. The cloned duck FSH-£] cDNA contains 1909-bp nucleotides including 396-bp of open-reading frame and 1491-bp of 3¡¬-untranslational region. The open-reading frame encodes a 131-amino acid protein with a putative 20-amino acid signal peptide and a putative 111-amino acid mature protein. The deduced amino acid sequence shows a remarkable similarity (94-98%) to those of other avian FSH-£] subunits; while it exhibits lower similarities with those of turtles (82-84%), mammals (63-71%), and amphibians (53-57%). The structural model analysis of duck FSH suggests that the cysteine-knot and £]-strands for maintaining the specific structural frame, and the "seat-belt" loop for specific binding to FSH receptor have been conserved in tetrapodian FSH-£]s. ? 2006 Elsevier Inc. All rights reserved.cDNA CloningDuckFollicle-stimulating hormone (FSH)Pituitary glandStructural model analysisjournal article10.1016/j.ygcen.2006.03.0132-s2.0-33747870231https://www.scopus.com/inward/record.uri?eid=2-s2.0-33747870231&doi=10.1016%2fj.ygcen.2006.03.013&partnerID=40&md5=86957410c7b19aa436852cc4a79e7bf9