Nain ATseng Y.-TWei S.-CPeriasamy A.PHuang C.-CTseng F.-GHUAN-TSUNG CHANG2021-08-032021-08-0320203043894https://www.scopus.com/inward/record.uri?eid=2-s2.0-85076840062&doi=10.1016%2fj.jhazmat.2019.121821&partnerID=40&md5=969f475ebad01a8eaf33c544cc97dd79https://scholars.lib.ntu.edu.tw/handle/123456789/575772We have prepared copper nanoclusters (Cu NCs) in the presence of bovine serum albumin (BSA) and 1,3-propanedithiol (PDT). The PDT/BSA–Cu NCs possess great activities against different types of bacteria, including non-multidrug-resistant bacteria (Escherichia coli, Salmonella Enteritidis, Pseudomonas aeruginosa, and Staphylococcus aureus) and multidrug-resistant bacteria (methicillin-resistant S. aureus). Their minimal inhibitory concentration (MIC) values are at least 242-fold and 10-fold lower than that of the free PDT and BSA–Cu NCs, respectively. The PDT/BSA–Cu NCs are strongly bound to the bacterial membrane, in which they induce the generation of ascorbyl (Asc[rad]) and perhydroxyl (HOO[rad]) radicals that result in disruption of their membrane integrity. At a concentration of 100-fold higher than their MIC for Escherichia coli, the PDT/BSA–Cu NCs exhibit negligible cytotoxicity towards the tested mammalian cells and show insignificant hemolysis. We have further demonstrated that low-cost PDT/BSA–Cu NCs-coated carbon fiber fabrics (CFFs) are effective against antibacterial growth, showing their great potential for antifouling applications. ? 2019 Elsevier B.V.Carbon fibers; Escherichia coli; Free radicals; Mammals; Microwave integrated circuits; Nanoclusters; Proteins; Salmonella; Textiles; Anti-bacterial activity; Antibacterial textiles; Bovine serum albumins; Copper nanoclusters; Minimal inhibitory concentration; Protein templates; Pseudomonas aeruginosa; Salmonella enteritidis; Copper compounds; 1,3 propanedithiol; antibiotic agent; bovine serum albumin; carbon fiber; copper; thiol derivative; 1,3-propanedithiol; antiinfective agent; copper; nanomaterial; propane; thiol derivative; antimicrobial activity; bacterium; chemical compound; cluster analysis; copper; free radical; ligand; nanoparticle; protein; antibacterial activity; bacterial growth; bacterial membrane; controlled study; Escherichia coli; matrix-assisted laser desorption-ionization mass spectrometry; minimum inhibitory concentration; nonhuman; photoluminescence; Pseudomonas aeruginosa; Salmonella enterica serovar Enteritidis; Staphylococcus aureus; chemistry; drug effect; Gram negative bacterium; Gram positive bacterium; microbial sensitivity test; microbial viability; microbiology; Bacteria (microorganisms); Bovinae; Escherichia coli; Mammalia; Pseudomonas aeruginosa; Salmonella enteritidis; Staphylococcus aureus; Anti-Bacterial Agents; Carbon Fiber; Copper; Gram-Negative Bacteria; Gram-Positive Bacteria; Microbial Sensitivity Tests; Microbial Viability; Nanostructures; Propane; Serum Albumin, Bovine; Sulfhydryl Compoundsjournal article10.1016/j.jhazmat.2019.121821318791162-s2.0-85076840062