2011-08-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/692384Abstract: The understanding of molecular mechanism of seed germination and dormancy of Arabidopsis under cold stratification, after-ripening, and high temperature has reached great progress in recent years. However, Arabidopsis seeds do not have deep dormancy when mature which is experienced by many tree seeds requiring the treatment of warm and cold stratifications. Prunus campanulata has thick endocarp, residual endosperm and a thin seed coat covering a huge embryo. The most efficient method to break the Prunus seed dormancy is application of a combination of four weeks warm stratification and followed by eight weeks of cold stratification. Even though we have the knowledge of cold stratification can promote the synthesis of bioactive GAs from the study of Arabidopsis, there is no documentation of the molecular changes or gene network of warm stratification. It will be very interesting to learn why warm treatment is required for the Prunus seeds in order to get full germination. We will apply subtractive suppressi on hybridization to study positive and negative regulators of ABA signal transduction pathway in the warm stratification and positive and negative regulators of GA signal transduction pathway in the cold stratification. These gene network s will be used to elucidate the mechanism especially of warm treatment in the breaking of deep seed dormancy of Prunus seeds.Prunus campanulatasubtractive suppression hybridizationwarm and