2006-01-012024-05-15https://scholars.lib.ntu.edu.tw/handle/123456789/663270摘要：研發出茄科重要病毒診斷鑑定晶片，並將研究成果轉移給國內防疫與檢疫單位使用。本計畫今年將執行下列 重要工作：(1)由GenBank收集並分析能感染茄科植物的cucumovirus的序列資料 ，設計並製備cucumovirus之廣效性引子對，以及能鑑定各種cucumovirus之單股寡 核酸探針。(2)以尼龍膜DIG系統及CMV cDNA株，測試所製備的核酸探針之專一 性與靈敏度。(3)以玻片螢光系統及CMV cDNA株，測試所製備的核酸探針之專一 性與靈敏度。(4)以番茄病株為材料，比較兩種不同的診斷鑑定晶片系統的效果。<br> Abstract: In order to develop an identification biochip for tomato viruses and provide the chip to the authorities of plant inspection and quarantine, the following works will be executed this year. (1) After collecting and analyzing the sequences of cucumoviruses, the genusspecific degenerate primers and oligonucleotide probes of the viruses will be designed and prepared. (2) By meaning of cDNA clones of CMV and DIG-membrane system, the specificity and sensitivity of the probes will be tested. (3) By meaning of cDNA clones of CMV and fluorescence-glass system, the specificity and sensitivity of the probes will be tested. (4) The efficiency of two biochip systems will be evaluated by cucumovirusinfected tomato plants.生物晶片μCE/ECD晶片聚合&#37238連鎖反應核酸晶片微流體檢測晶片磁性奈米微顆粒biochipμCE/ECD chipPolymerase chain reactionDNA chipmicrofluidic biochipmagnetic nano-particles