2014-06-012024-05-14https://scholars.lib.ntu.edu.tw/handle/123456789/660876摘要： 生物支架提供組織形成時的一個平台。傳統上組織載體常為非自體性材料、包含金屬物質、骨水泥、聚合物及動物組織等，造成了生物支架的生物適應性及實用性上之限制。因此在本研究中，我們將使用哺乳動物體液中含量豐富之自體血漿白蛋白做為原料，並利用細菌源之轉谷氨酰胺酶將自體血漿白蛋白聚合成為組織載體。利用掃描式電子顯微鏡、材料試驗機等檢測其三度空間立體形態學及其物理機械特性。之後，利用大鼠間葉幹細胞測試其生物相容性及各項 細胞分化特性。第二年之計畫中，我們將先後以大鼠自體血漿白蛋白、大鼠異體血漿白蛋白、不同動物體血漿白蛋白製作載體並進行實驗。第三年之計畫中，我們將以大動物為實驗模式製作載體並進行實驗，並對未來人體實驗預作準備 <br> Abstract: Tissue scaffold provides a platform for tissue formation. However, tissue scaffolds are non-autologous, consisting of metals, cements, polymers, and animal tissues with a confined biocompatibility and application. Therefore, this study presents a processing method to generate an autologous tissue scaffold from albumin, the major protein in mammalian blood. Albumins were polymerized into an albumin polymer by microbial transglutaminase, and further processing by freeze-drying molding to form an albumin tissue scaffold. Scanning electron microscopy and material testing analyses demonstrated that albumin tissue scaffold has porous structure, moderate mechanical strength, and resilient property. Using three-dimensional (3-D) tissue culture of rat mesenchymal stem cells (MSCs) as a model, we will test their biocompatibility, cell differentiation and possible clinical applications. During the 2nd year’s project, we will perform the animal study on nude mice, autognous albumin scaffold animal study on Witar rat, allogenous albumin scaffold animal study on Witar rat and then xenogenous albumin scaffold animal study on Witar rat. To expand its possible clinical apllictions. During the 3rd year’s project, the possible applications on Larger animla (such as Pig or sheep) will be performe and then preparation for human study will be arranged.白蛋白自體移植再生醫學幹細胞組織載體組織工程AlbuminAutograftRegenerative medicineStem cellsTissue scaffoldTissue engineering