Chao, Pei-MinPei-MinChaoChao, Che-YiChe-YiChaoLin, Fu-JungFu-JungLinHuang, Ching-JangChing-JangHuang2009-07-232018-07-062009-07-232018-07-06200100223166http://ntur.lib.ntu.edu.tw//handle/246246/162612https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035212603&doi=10.1093%2fjn%2f131.12.3166&partnerID=40&md5=56212ec7c9dcf4245bd1e7e66ef9120eOxidized LDL (oxLDL) and its component hydroxy fatty acids were shown to activate peroxisome proliferator-activating receptor α (PPARα) and γ (PPARγ). To test the hypothesis that lipid oxidation products in oxidized frying oil (OFO) can activate PPARα and up-regulate its target genes, a feeding experiment and a transactivation experiment were conducted. Based on a 2 × 2 factorial design, four groups of Sprague-Dawley male weanling rats were fed diets containing either high (20 g/100 g, HO and HF) or low (5 g/100 g, LO and LF) levels of oxidized frying soybean oil (HO and LO) or fresh soybean oil (HF and LF) for 6 wk. The OFO sample was prepared by frying wheat dough sheets in soybean oil at 205 ± 5°C for 24 h. OFO dose dependently and significantly increased (P < 0.05) mRNA of acyl-CoA oxidase (ACO) and cytochrome P450 4A1(CYP4A1) in liver of rats. Dietary OFO also dose dependently increased liver microsomal CYP4A protein (P < 0,05). The activity of hepatic ACO of the HO group was sixfold that of the HF group (P < 0.05). Plasma total lipids, liver triglycerides, cholesterol and total lipids were reduced in rats fed the LO and HO diets (P < 0.05). Through the ligand binding domain of PPARα, the hydrolyzed OFO enhanced the expression of alkaline phosphatase (ALP) reporter gene to a significantly greater extent (P < 0.05) than the hydrolyzed fresh soybean oil in a transactivation assay using a clone of CHO K1 cells stably expressing Gal4-PPARα chimeric receptor and UAS4-ALP reporter. The results support our hypothesis that dietary OFO, by activating PPARα, up-regulates the expression of PPARα downstream genes and alters lipid metabolism in rats.application/pdf360629 bytesapplication/pdfen-USAcyl-CoA oxidase; CYP4A1; Oxidized frying oil; PPARα; Ratsacyl coenzyme A oxidase; alkaline phosphatase; chimeric protein; cholesterol; cytochrome P450; hydroxy fatty acid; lipid; oxidized low density lipoprotein; peroxisome proliferator activated receptor gamma; soybean oil; triacylglycerol; animal cell; animal tissue; article; controlled study; dietary intake; DNA flanking region; gene activity; gene expression regulation; gene targeting; ligand binding; lipid metabolism; liver microsome; male; nonhuman; rat; receptor upregulation; reporter gene; Animalia; Glycine max; Rodentia; Siganus; Triticum aestivumjournal article117398612-s2.0-0035212603http://ntur.lib.ntu.edu.tw/bitstream/246246/162612/1/21.pdf