2003-08-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/693760摘要：靈芝與巴西洋菇均含有抑制腫瘤與免疫調節活性的多醣，其活性分佈在數個多醣區分中，其中以具有 (1→6)-β-D-葡萄糖基之 (1→3)-β-D-聚葡萄醣的化學性質與活性有較清楚的研究。本子計畫擬配合其他研究群，使用也含有免疫調節功能多醣的豆科植物－黑豆與黃耆萃取液為發酵基質，探討豆科與靈芝或巴西洋菇活性多醣的加乘作用。由本研究群以往研究知道，發酵基質與發酵條件會直接影響靈芝菌絲發酵產品的多醣生成量與組成特徵，並預期發酵過程中菇菌的多醣分解酵素的作用，可部分降解豆科多醣，增加豆科多醣的可溶性。本計畫擬分三年進行發酵複合產品的多醣性質探討，第一年，將以上述的聚葡萄醣為起始指標，分析發酵基質對多醣生成量與組成的影響，並配合免疫活性子計畫，建立多醣組成特徵與活性的相關性，並尋找更適合的多醣指標或第二指標；第二年，同樣配合其他子計畫以活性為指標，探討發酵條件對多醣組成特徵的影響，並定出在最適發酵條件下，所生產多醣的組成特徵後，再探討分離具活性多醣的方法；第三年，以最適條件生產之發酵產品，針對二至三個主要活性多醣區分，進行分離與純化，並分析其化學組成與分子量，並將純化或部分純化的多醣區分以反應曲面設<br> Abstract: Varieties of polysaccharides from Ganoderma lucidum Krast and Agaricus blazei Murill, both Basidiomycete fungi, have the ability to enhance the immune system and to inhibit growth of tumor. The (1→3)-β-D-glucan bearing (1→6)-β-glucosyl branches is the well-known and major component of the active polysaccharide. The goal of this study is to evaluate activity synergistic effects of polysaccharides of Leguminosae plants, black soybean (Glycine max) and Astragalus membranaceus Bunge and G. lucidum or A. blazei. The extracts of black soybean and A. membranaceus are used as mycelium culture medium to incorporate their active polysaccharides into fermented products of G. lucidum or A. blazei. According our previous studies, the medium composition and condition of fermentation significantly altered the composition characteristics and quantity of G. lucidum polysaccharides. The solubility of legume polysaccharides is expected to increase through the function of polysaccharide degradation enzymes in the fermentation process. The tentative schedule has been set as followings: First year, the works focus on the composition difference caused by using legume extracts. The (1→3)-β-D-glucan mentioned is used as initiation index to study sugar component changes of the polysaccharides. A better index or second index will choose according the results of immune modulating activity. The works of second year focus on the quantity of polysaccharide indices and composition difference caused by fermentation conditions. The separation methods will develop to isolated potential active polysaccharides from mycelium culture from optimized condition according immunomodulating ability. A scale-up separation will conduct in third year to isolated 2 to 3 major fractions of active polysaccharides. The chemical composition and molecular weight of purified active polysaccharides will examine. A response surface method will employee to evaluate the synergistic effects of immune activity by reconstituted active polysaccharide fractions. The water-solubility and stability of solution will also examine.靈芝巴西洋菇黑豆黃耆多醣(1→3)-β-D-聚葡萄醣Ganoderma lucidum KrastAgaricus blazei MurillGlycine maxblack soybeanAstragalus membranaceus Bungepolysaccharides(1→3)-β-D-glucan