2002-08-012024-05-18https://scholars.lib.ntu.edu.tw/handle/123456789/698089摘要：本研究是利用孔雀魚來發展及建立一整合型的水生生物測試方法，用於檢測水中的環境雌性激素。在1、2或3周時間下，雄孔雀魚分別暴露壬基苯酚10,60或150μg/L。在各暴露時間的雌二醇組及壬基苯酚150μg/L暴露組雄孔雀魚的卵黃前質誘發均與其控制組有顯著差異。其誘發反應持續增強至14天達到一個明顯的高峰。另外，在21天暴露下壬基苯酚10μg/L暴露組雄孔雀魚的卵黃前質誘發也與其控制組有顯著差異。雄孔雀魚生殖腺成熟指數呈現隨暴露時間增長而降低的現象，但是其與壬基苯酚劑量並無明顯劑量與反應關係。另外，除了21天的壬基苯酚60μg/L暴露組外，利用Resazurin reduction assay來測量雄孔雀魚的精子代謝活性可發現，不同暴露組與其控制組沒有顯著不同。由本研究結果可發現雄孔雀魚的卵黃前質是較敏感的生物指標反應暴露環境雌性激素。<br> Abstract: Adult male guppies (Poecilia reticulata) were exposed to 4-nonylphenol (NP) at nominal concentrations of 10, 60 and 150 μg/L for 7, 14 or 21 days of treatment. Significant induction of vitellogenin (VTG) were found in male guppies exposed to 1μg/L 17βestradiol, 60μg/L and 150μg/L NP after 7, 14 and 21 days of treatment and were already at their maximum induction after 14 days of exposure treatment. On the other hand, significant induction of VTG in male guppies exposed to 10μg/L NP was only observed after 21 days exposure. A time-dependent tendency for reduction of gonadosomatic index (GSI) in male guppies treated with 17β-estradiol or NP was observed, no concentration- dependent tendency of decreased GSI in male guppies was detected. There were also no differences in sperm metabolic activities measured by resazurin reduction assay of male fish treated with different concentrations of NP during different exposure periods, except for activity of guppy treated with 60μg/L NP after 21 days exposure . Preliminary data presented in this study strongly suggest that measurement of plasma VTG induction in male guppies is a suitable and rather sensitive indicator of exposure to estrogenic chemicals.內分泌干擾物質生物指標環境雌性激素卵黃前質孔雀魚Endocrine disruptorbiomarkerenvironmental estrogenvitellogeninguppy