Wu, Yong-PeiYong-PeiWuSHU-MEI WANGChang, Yu-ChiYu-ChiChangHo, ChiChiHoHsu, Yu-ChiaYu-ChiaHsu2022-04-252022-04-25202116616596https://www.scopus.com/inward/record.uri?eid=2-s2.0-85120971320&doi=10.3390%2fijms222413365&partnerID=40&md5=2345034970edfa10ca3aac902af22383https://scholars.lib.ntu.edu.tw/handle/123456789/605912Flash flooding is a major environmental stressor affecting rice production worldwide. DT3 is a drought-tolerant, recurrent parent with a good yield, edible quality, and agronomic traits akin to those of an elite Taiwanese variety, Taiken9 (TK9). Progenies carrying Sub1A can enhance submergence stress tolerance and can be selected using the marker-assisted backcross (MAB) breeding method. For foreground selection, Sub1A and SubAB1 were utilized as markers on the BC2 F1, BC3 F1, and BC3 F2 generations to select the submergence-tolerant gene, Sub1A. Background selection was performed in the Sub1A-BC3 F2 genotypes, and the percentages of recurrent parent recovery within individuals ranged from 84.7–99.55%. BC3 F3 genotypes (N = 100) were evaluated for agronomic traits, yield, and eating quality. Four of the eleven BC3 F4 lines showed good yield, yield component, grain, and eating quality. Four BC3 F4 lines, SU39, SU40, SU89, and SU92, exhibited desirable agronomic traits, including grain quality and palatability, consistent with those of DT3. These genotypes displayed a high survival rate between 92 and 96%, much better compared with DT3 with 64%, and demonstrated better drought tolerance compared to IR64 and IR96321-345-240. This study provides an efficient and precise MAB strategy for developing climate-resilient rice varieties with good grain quality for flood-prone regions. ? 2021 by the authors. Licensee MDPI, Basel, Switzerland.Background selectionDrought toleranceForeground selectionJaponica DT3Marker-assisted backcrossSubmergence toleranceadultagronomic traitarticlebackground selectionclimatecontrolled studydrought toleranceeatingfemalegenetic markergenotypehumanJaponica ricemajor clinical studymalenonhumanpalatabilityremissionsurvival rateadaptationdroughtfloodinggeneticsmetabolismOryzaphenotypephysiological stressplant breedingplant geneproceduresplant proteinAdaptation, PhysiologicalDroughtsFloodsGenes, PlantGenotypePhenotypePlant BreedingPlant ProteinsStress, Physiological[SDGs]SDG13[SDGs]SDG15journal article10.3390/ijms222413365349481652-s2.0-85120971320