Production of a high molecular weight levan by Bacillus paralicheniformis, an industrially and agriculturally important isolate from the buffalo grass rhizosphere

Nasir, AnamAnamNasirAhmad, WaqarWaqarAhmadSattar, FazalFazalSattarAshfaq, IramIramAshfaqLindemann, Stephen RStephen RLindemannMING-HSU CHENVan den Ende, WimWimVan den EndeӦner, Ebru ToksoyEbru ToksoyӦnerKirtel, OnurOnurKirtelKhaliq, ShaziaShaziaKhaliqGhauri, Muhammad AMuhammad AGhauriAnwar, Munir AMunir AAnwar2023-03-312023-03-312022-090003-6072https://scholars.lib.ntu.edu.tw/handle/123456789/629943A new exopolysaccharide (EPS) producing Gram-positive bacterium was isolated from the rhizosphere of Bouteloua dactyloides (buffalo grass) and its EPS product was structurally characterized. The isolate, designated as LB1-1A, was identified as Bacillus paralicheniformis based on 16S rRNA gene sequence and phylogenetic tree analysis. The EPS produced by LB1-1A was identified as a levan, having β(2 → 6) linked backbone with β(2 → 1) linkages at the branch points (4.66%). The isolate LB1-1A yielded large amount (~ 42 g/l) of levan having high weight average molecular weight (Mw) of 5.517 × 107 Da. The relatively low degree of branching and high molecular weight of this levan makes B. paralicheniformis LB1-1A a promising candidate for industrial applications.enBacillus paralicheniformis; Exopolysaccharide; Fructan; Fructooligosaccharide; LevanProduction of a high molecular weight levan by Bacillus paralicheniformis, an industrially and agriculturally important isolate from the buffalo grass rhizospherejournal article10.1007/s10482-022-01760-6358408142-s2.0-85134307513WOS:000825922600001https://api.elsevier.com/content/abstract/scopus_id/85134307513