宋孔彬臺灣大學:電機工程學研究所巢國山Chao, Guo-ShanGuo-ShanChao2010-07-012018-07-062010-07-012018-07-062008U0001-1007200817273600http://ntur.lib.ntu.edu.tw//handle/246246/187980逆散射光譜對於細胞結構與折射率的變化非常的敏感，因此，若能建立一套模擬工具，將逆散射光譜與細胞結構以及折射率變化量之間的關係量化，將有機會實現上皮細胞非侵入式癌症檢測。 本論文主要是利用有限差分時域（FDTD）法來建立模擬工具，並加入高斯脈衝波，以達到模擬寬波長範圍的散射光，FDTD是一套彈性且實用的數值方法來解Maxwell’s equation，其適用於任意形狀與電介質的物體。可藉由調整細胞模擬參數來觀察逆散射光譜的變化，藉此找出逆散射光譜與細胞結構以及折射率變化之間的關係。 利用Mie theory來驗證在均質圓球條件下的模擬結果，並且討論不同空間取樣率對於模擬結果準確度、消耗時間與記憶體使用量的影響。最後模擬數個不同條件的細胞核散射光譜，觀察其逆散射光譜的變化，確實會受到細胞核結構與折射率變化量的影響。Backscattering is very sensitive to structure and refraction index variation of cell. Hence, if I can build a simulation tool to find relationship between backscattering and structure and refraction index variation of cell, and then, it would be potential to achieve non-invasive epithelium cancer diagnosis. In this thesis, I use finite difference time domain (FDTD) method to build simulation tool, and use Gaussian pulsed for achieving simulation light scattering over broad wavelength ranges. FDTD is a flexible and powerful method to solve Maxwell’s equation. FDTD is suitable for dielectric objects of arbitrary shape. By change the simulation parameter of cell to observe backscattering, and investigate the relationship between backscattering and structure and refraction index variation of cell. To verify FDTD simulation result of uniform sphere by Mie theory, and discuss the influence of special sampling on accuracy, elapsed time and memory size. At last, I simulate several different nuclei, and observe the influence of structure and refraction index variation of cell on backscattering. The backscattering is certainly influenced by structure and refraction index variation of cell.口試委員會審定書…………………………………………………… i謝…………………………………………………………………… ii文摘要…………………………………………………………… iii文摘要…………………………………………………………… iv一章 簡介………………………………………………………… 1 1-1 研究動機…………………………………………………… 1 1-2 研究目標…………………………………………………… 4二章 研究方法…………………………………………………… 5 2-1 The Yee Algorithm………………………………………… 5 2-2 Total Field/Scattered Field Formulation………… 11 2-3 Light Source……………………………………………… 12 2-4 PML Boundary Condition………………………………… 14 2-5 Near-to-Far-Field Transform………………………… 22三章 程式驗證與分析………………………………………… 26 3-1 Comparisons with Mie Theory………………………… 26 3-2 BroadBand Result………………………………………… 31 3-3 Analysis of different grid spacing………………… 33四章 微米圓球的散射光譜……………………………………… 37 4-1 簡介與實驗方法…………………………………………… 37 4-2 實驗結果…………………………………………………… 39 4-3 與模擬結果比較…………………………………………… 41五章 細胞核散射光譜…………………………………………… 43六章 討論………………………………………………………… 48六章 結論與未來工作…………………………………………… 53考文獻……………………………………………………………… 541575500 bytesapplication/pdfen-US有限差分時域法逆散射光譜細胞結構折射率變化量Finite Difference Time DomainBackscatteringCell StructureRefraction Index VariationMie theory[SDGs]SDG3thesishttp://ntur.lib.ntu.edu.tw/bitstream/246246/187980/1/ntu-97-R95921106-1.pdf