2008-07-172024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/694421摘要：反芻動物的瘤胃是一個厭氣的特殊環境。反芻動物本身的消化道因缺乏纖維分解酵素，必須仰賴消化道中的瘤胃微生物分泌相關酵素幫助消化分解，以供動物本身能量的來源，因此瘤胃微生物演化成一具有高度分解纖維素和半纖維素能力的族群，成為目前纖維分解酵素的潛力分離源。瘤胃微生物可利用的受質種類相當廣泛，除纖維素以外，並涵蓋各式木質纖維素廢棄物，如稻桿、麥桿，以及木聚糖等植物結構性多醣。因此，瘤胃微生物為分離高活性纖維分解酵素基因之極佳分離源。而本土水牛具有耐粗飼之特性，故為纖維分解酵素的開發潛力來源。本研究擬自台灣水牛瘤胃微生物中，篩選分離具有纖維分解能力之菌株，經由建構其cDNA library後，選殖其纖維分解酵素基因，包含纖維素&#37238;（cellulase）與木聚糖&#37238;（xylanase）等，並將纖維分解酵素基因建構於酵母菌表達系統中，以進行分解酵素的大量產製。研究成果期能應用於飼料、農業廢棄物處理、生質酒精之開發與產製等用途。<br> Abstract: The rumen is widely considered to be a large anaerobic vat. Ruminants themselves do not produce fiber-degrading enzymes, but they harbor a complex of symbiotic microorganisms, including bacteria, fungi, and protozoa, which are responsible for breakdown of fiber. The host provides the microbes with a suitable habitat for growth, and the microbes supply protein, vitamins, and short-chain organic acids for the host. Ruminal microbes are able to degrade the most-resistant plant cell-wall polymers, thus, the rumen microbial population represents a rich and underutilized source of fibrolytic enzymes with tremendous potential for industrial and agricultural applications. As Taiwan buffaloes are known to digest crude fiber in better way, therefore, they could be a rich potential source of novel enzymes discovery. This research aims at screening the ruminal anaerobic microbes that possess the ability to degrade fiber, including cellulose and xylan. The genes of the fibrolytic enzyme will be cloned and expressed in high efficiency expression system. The application of the recombinant enzymes will also be estimated.台灣水牛瘤胃微生物纖維分解酵素Taiwan water buffaloruminal microbefibrolytic enzyme