楊泮池2006-08-312018-07-112006-08-312018-07-112004http://ntur.lib.ntu.edu.tw//handle/246246/29788微陣列是發展及應用較為成熟的生物晶片技術之一，此晶片技術已廣泛的應用於各領域 的基礎研究，亦將直接或間接的應用於疾病之診斷、分類、疾病之預後評估，並改善疾病之 治療。由於基因晶片的設備及成本高昂，導致此技術無法廣為應用於各領域之研究。本計劃 之目的為設立微陣列核心實驗室及基因庫，將以合理價格提供高品質之人類及老鼠基因微陣 列晶片實驗服務，包括核酸標記、雜交反應、呈色偵測、影像分析、數值擷取及叢集分析， 另外並代為製作各式晶片。核心實驗室將依最大工作能量，提供參與基因體醫學國家型科技 計畫及其他國科會研究計畫之各學術及研究機構研究人員申請使用。 本核心實驗室已於台大醫學院附設醫院設置三年有餘，製作超過一千五百片的人類、老 鼠及代製晶片，並提供醫學校區相關的研究諮詢與教育訓練。去年度受國科會補助成立「微 陣列基因體醫學核心實驗室」，並立即提供全國性的微陣列服務，為國內能完整提供基因微 陣列服務的核心實驗室之一。目前已提供台大、陽明、中山、長庚、中山醫學等大學超過三 百片人類、老鼠及代製晶片，後續登記要求服務的仍有數百片，將依登記先後及實驗室製作 容量提供服務。Microarray technology is the central platform for functional genomics and translational research in the post-genomic era. Our main goal is to establish an up-to-date microarray core facility, dedicated to provide high quality microarrays, technical supports and services to researchers in genomic medicine nationwide. Access to this high quality microarrays will allow our researchers to be on the cutting edge of genetic research, focus on microarray-based gene expression profiling to explore the pathogenic mechanism of human diseases. The microarray technology is particularly important to clinical medicine in post-genomic era. It will provide high throughput and valuable insights into differences in an individual’s disease as compared with constitutional mRNA expression. The microarray information can provide tissue-specific disease signatures and therefore, can improve disease diagnosis, disease classification, prognosis evaluation, identify biomarkers, individualized patient treatment and improve treatment outcome. Although the cDNA microarray is extremely powerful, the current application is still limited. The major obstacles are its high cost and difficulty in maintaining good quality membrane and experiment. The purpose of this project presented here is to establish the Core Lab and ESTs library for high quality service of membrane-based cDNA microarray under the reasonable price. The core facility will provide services including target labeling, microarray hybridization, colorimetric detection, image digitization, and cluster analysis. In addition, the services of custom-made chips including membrane- and glass-format microarray are also provided. According to the work capacity, microarray core facility will open for the investigators of academic and research institution participated in National Research Program of Genomic Medicine to apply. The core facility has been established in National Taiwan University Hospital, National Taiwan University College of Medicine for more than three years and manufactured more than 1,500 pieces of microarray membranes including human, mouse, and custom-made chips. Also, the Core Lab offered related research consultation and education training to medical campus. National Science Council supports our core facility last year to set up the “Microarray Core Facility for Genomic Medicine”, which offers countrywide microarray services right away. The microarray core facility has provided complete microarray services for dozens of institutions, including the researchers of Taiwan University, Chung-Shan, Yang-Ming University, Chang-Geng University, Chung-Shan Medical University, and so on, for more than 300 pieces of microarray membranes including human, mouse, and custom-made chips. There are still hundreds of chips waiting for preparation. We will provide services by the enrolled order and work capacity.application/pdf295591 bytesapplication/pdfzh-TW國立臺灣大學醫學院內科微陣列Microarray[SDGs]SDG3reporthttp://ntur.lib.ntu.edu.tw/bitstream/246246/29788/1/913112P002017Y.pdf