Lee, Hsuan-ShuHsuan-ShuLeeGUAN-TARN HUANGJIN-CHUAN SHEUChiou, Ling-LingLing-LingChiouHorng M.-C.Lai, Ming-YangMing-YangLaiDING-SHINN CHENSHENG-CHUNG LEE2021-02-022021-02-0219950006-291Xhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-0029148493&doi=10.1006%2fbbrc.1995.1758&partnerID=40&md5=8a78ab59bcfb1f84c21d0516283a1cafhttps://scholars.lib.ntu.edu.tw/handle/123456789/545803Hepatocyte growth factor (HGF) is a cytokine with pleiotropic effects on many different cell types. Its biological activities depend on the disulfide-linked alpha beta heterodimeric molecule. To study the functions of the beta-chain of HGF, insect cell-expressed HGFs prepared from nested deletions from C-terminus of beta-chain were studied for their biological activities and ligand-binding functions. The results demonstrated that the functions of HGF are dependent on the intactness of its beta-chain. The loss of HGF activities is correlated with the progressive deletion of beta-chain. There is not a single critical domain in the beta-chain that is essential for the functions of HGF. We propose that beta-chain may act to stabilize the ligand-receptor binding or contribute to the proper conformation of HGF to interact with its receptor.journal article10.1006/bbrc.1995.175875392542-s2.0-0029148493