2009-08-012024-05-13https://scholars.lib.ntu.edu.tw/handle/123456789/652221摘要：由於導電高分子，例如：Poly(3,4-ethylenedioxythiophene) (簡稱PEDOT)所具有不同且特殊的光電特性，使得以這類化合物修飾的電極多年來在電致色變元件、薄膜電池、光電池及感測薄膜等光電研究領域倍受重視。然而，隨著愈來愈多研究結果顯示修飾電極對特定的生化物質具有良好的電催化能力，因此修飾電極在生醫感測的應用更是成了熱門的研究題目。本計畫除了將致力於導電高分子PEDOT酵素修飾電極的生化感測研究之外，亦將結合微機電製程優點，以『利用酵素固定法研究微流道葡萄糖感測晶片』為主題來進行深入探討。 本計畫預定以兩年的時間完成相關的研究工作。第一年的研究重點將以PEDOT固定葡萄糖氧化酵素於微型柵狀電極之研究；第二年的研究重點則為以PDMS建構微流道晶片並整合PEDOT酵素電極之研究。因此，第一年之工作項目包括：微型柵狀電極之製備、製備好之PEDOT酵素電極以SEM對其奈米級結構進行分析、以及掌握酵素修飾電極對葡萄糖的最佳感測環境及條件。第二年之工作項目則包括：流道母模之製備及PDMS流道製作，以及生化感測晶片之感測參數與感測行為比較。我們預期本計畫除了能促使整合型生化感測晶片技術蓬勃發展之外，更能使化學修飾電極的電化學奈米製程技術趨向完備，且具有提升現有光電材料鍍膜使用價值的附加功能。因此本計畫堪稱一份結合『生醫感測』、『微機電製程』與『酵素薄膜』等重點科技的研究藍圖。 <br> Abstract: Due to the unique electro-optical properties of conducting polymers, such as Poly(3,4-ethylenedioxythiophene), or PEDOT, the modified electrodes of these materials have been investigated for applications in electrochromic devices, thin-film batteries, photoelectrochemical cells, and sensing thin films for many years. Recently, however, these active materials have become very popular on the research for the electrochemical biosensors after successful demonstrations of the electrocatalytic capabilities for specific bimolecules. This proposal not only will devote to the sensing study using PEDOT-enzyme modified electrodes, but also intend to take advantages of micro-electro-mechanical-process and further exploring the topic of “On the study of microfluidic glucose sensing chip by enzyme immobilization.” We plan to spend two years executing the proposal. It is planned to immobilize glucose oxidase on the interdigitated microelectrode through the entrapment of PEDOT during the first year. The integration of PEDOT-enzyme film in a micro-channel constructed by PDMS will be the focus of the second year in developing the microfluidic sensing chip. Accordingly, the tasks to be completed for the first year include fabrication of interdigitated microelectrode, the nano-structured analysis of PEDOT-enzyme film by SEM, and finding best sensing conditions of enzyme film for glucose. In the second year, the researches will be directed torward the fabrications of the micro-channel mold and micro-channel by PDMS. The comparison between sensing parameters and sensing performance of the sensing chip will be done. It’s our expectation that not only the integrated biosensing chip techniques but also the electrochemical nano-processes using a chemically modified electrode (CME) technology can be promoted after the completion of this proposal. Furthermore, it will enhance the add-value of electro-optically active PEDOT thin films. Consequently, this proposal can be viewed as a research blueprint that integrates the currently highlighted technologies with biomedical sensing, micro-electro-mechanical-process, and enzyme thin films.化學修飾電極酵素微機電製程微流體元件感測晶片Chemically modified electrode (CME)enzymemicro-electro-mechanical-processmicrofluidic devicesensing chip