Zuo, LiLiZuoWEI-TING KUOCao, FengFengCaoChanez-Paredes, Sandra DSandra DChanez-ParedesZeve, DanielDanielZeveMannam, PrabhathPrabhathMannamJean-François, LéaLéaJean-FrançoisDay, AnneAnneDayVallen Graham, WWVallen GrahamSweat, Yan YYan YSweatShashikanth, NiteshNiteshShashikanthBreault, David TDavid TBreaultTurner, Jerrold RJerrold RTurner2022-11-102022-11-1020230017-57491468-3288https://scholars.lib.ntu.edu.tw/handle/123456789/624556Intestinal barrier loss is a Crohn's disease (CD) risk factor. This may be related to increased expression and enzymatic activation of myosin light chain kinase 1 (MLCK1), which increases intestinal paracellular permeability and correlates with CD severity. Moreover, preclinical studies have shown that MLCK1 recruitment to cell junctions is required for tumour necrosis factor (TNF)-induced barrier loss as well as experimental inflammatory bowel disease progression. We sought to define mechanisms of MLCK1 recruitment and to target this process pharmacologically.enIBD basic research; TNF; actin cytoskeleton; intestinal barrier function; intestinal stem celljournal article10.1136/gutjnl-2021-32653435537812WOS:000793935800001