生命科學院: 植物科學研究所指導教授: 謝旭亮羅翊郢Lo, Yi-YingYi-YingLo2017-03-032018-07-062017-03-032018-07-062015http://ntur.lib.ntu.edu.tw//handle/246246/274957植物光形態發生 (photomorphogenesis) 受光及賀爾蒙 (phytohormones) 的共同調控。先前研究指出FAR-RED INSENSITIVE 219 (FIN219) 會和CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) 進行交互作用，而COP1 在光照下會從細胞核轉移至細胞質累積；此外，即使在黑暗下，FIN219大量表現會使COP1從細胞核轉移到細胞質。FIN219/JASMONATE RESISTANT 1 (JAR1) 亦可催化 JA-isoleucine (JA-Ile) 生合成，並透過茉莉酸 (jasmonate) 訊息傳遞途徑之正調控者 CORONATINE INSENSITIVE 1 (COI1) 接收。然而，對光及茉莉酸訊息傳遞的整合研究仍未釐清透徹；另外COP1參與多種植物賀爾蒙的訊息傳遞，但是否參與在茉莉酸訊息傳遞途徑中亦是未知。故我們欲探究COP1在黑暗下的位置轉移是否透過受FIN219所調控的JA-Ile影響，並探討FIN219、COP1及COI1三者在此訊息整合中的調控關係。本研究中發現cop1-4突變體對於methyl-jasmonic acid (MeJA) 較不敏感，且COP1正調控受MeJA誘發的葉綠素生合成，並負調節花青素累積。此外，透過外表型檢測及轉錄和轉譯體表現分析得知COP1亦會參與在茉莉酸訊息傳遞途徑中，而COI1也會影響光訊息傳遞相關成員的表現。並使用共免疫沉澱法證實FIN219和COP1在黑暗下存在交互作用，且此交互作用會受到MeJA促進。另外值得注意的是MeJA的確會提升COP1在黑暗下於細胞質的累積，進而造成如光形態發生的表型。綜而言之，FIN219、COP1及COI1在光及茉莉酸訊息傳遞途徑中相輔作用，以調控植物生長發育。Photomorphogenic development of plants needs the integration of light and phytohormones. FAR-RED INSENSITIVE 219 (FIN219) interacted with CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) that was transferred from the nucleus to the cytoplasm in the light. Interestingly, FIN219 overexpression results in changes of COP1 subcellular localization from the nucleus to the cytoplasm even in the dark. FIN219/JASMONATE RESISTANT 1 (JAR1) catalyzes the synthesis of JA-isoleucine (JA-Ile), and JA-Ile is received by CORONATINE INSENSITIVE 1 (COI1). However, the molecular mechanism underlying the integration of light and jasmonate signaling pathways remains largely uncertain. Furthermore, COP1 has crosstalk with several phytohormones, but with jasmonate or not is still unknown. Thus, we wonder whether changes of COP1 subcellular locations from the nucleus to the cytoplasm under the dark are caused by FIN219-mediated JA-Ile. We are also interested in the relationship among FIN219, COP1 and COI1 in the integration of far-red light and jasmonate signaling. Here, we found that cop1-4 mutant is more insensitive to methyl-JA (MeJA) than Col-0. COP1 acts as a positive and a negative regulator in MeJA-promoted chlorophyll and anthocyanin accumulation, respectively. In addition, examination of phenotype, transcription and translation levels reveals that COP1 is involved in jasmonate signaling pathway and COI1 affects the protein levels of light signaling components. Furthermore, COP1 can interact with FIN219 under the dark, and this interaction is enhanced by MeJA. Strikingly, MeJA treatment can elevate the level of COP1 accumulation in the cytoplasm under the dark and result in the phenotype similar to the photomorphogenic development of seedlings. Taken together, FIN219, COP1, and COI1 coordinate far-red light and jasmonate signaling pathways, and play important roles in plant growth and development.2834179 bytesapplication/pdf論文公開時間: 2025/12/31論文使用權限: 同意有償授權(權利金給回饋學校)光茉莉酸COP1FIN219COI1阿拉伯芥幼苗發育LightJasmonatesCOIArabidopsis seedling developmentthesishttp://ntur.lib.ntu.edu.tw/bitstream/246246/274957/1/ntu-104-R01b42014-1.pdf