2020-08-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/676856摘要： 以生物性製劑取代或減少化學性農用製劑的使用量為全球農業永續發展的重點之一。要使生物性肥料/保護劑中的有益微生物在與宿主植物共生的過程中發揮最大功效，則需了解「環境－作物－共生菌」的交互作用機制，才能精準施用以達促進作物生長之目的，而這也正是生物性農用製劑使用時所面臨的挑戰之一。印度梨形孢真菌(Piriformospora indica, P. indica)為宿主種類廣泛的植物根部內共生真菌，其與水稻共生可助根系發展並顯著提高榖粒產量。我們的研究亦顯示印度梨形孢真菌可經茉莉酸訊息途徑誘導胰蛋白酶抑制因子活性，進而提升水稻對瘤野螟的抗性。印度梨形孢真菌為極具應用性之內共生真菌，了解環境因子對印度梨形孢真菌與植物共生的影響及機制，將有助於提升其與宿主作物共生的效能及穩定度，使其更具應用潛力。本計畫擬著重於探討環境氮營養源對印度梨形孢真菌與水稻共生效能的影響及生理與分子機制，另一方面，我們亦將分析水稻品種間氮素利用效率的差異對其與印度梨形孢真菌共生效率的影響。在此研究中我們將運用以影像分析為基礎的表型體系統觀察水稻的生長速度及生理指標。另將以轉錄體分析探討氮源影響印度梨形孢真菌與水稻共生效率之調控網絡。 Abstract Replacing or reducing the use of chemical fertilizers and pesticide with the application of bio-fertilizer and plant protection agent is a development trend in global agriculture. Understanding the interaction mechanism of "environment-crop-symbiotic bacteria" can help to precisely apply it into field for achieving the great efficiency of promoting crop growth. However, it still is a challenges for bio-fertilizer application. Piriformospora indica (P. indica) is an endophytic fungi and has broad spectrum of plant host. P. indica colonization will promote rice root growth and increase grain yield. Our study showed P. indica colonization also can enhanced trypsin inhibitor activity mediated jasmonic acid signaling and further increase the insect resistance against to Cnaphalocrocis medinalis Guenée. P. indica is a great candidate for developing to be a biofertilizer and also protection agent. Revealing the effects of environmental factors on the symbiosis of P. indica and plants will help to improve the colonization efficiency and stability. The project aims to understand the effects of environmental nitrogen sources on the symbiosis of P. indica and rice plants. Furthermore, the physiological and molecular mechanisms involved to the nitrogen-regulated colonization will be focused to study. In this study, the phenotyping system will be used to analyze plant growth and physiological characteristics. Transcriptome analysis will be used to explore the regulatory network of nitrogen -regulated symbiosis of P. indica and rice.印度梨形孢真菌內共生真菌水稻共生效率氮源Piriformospora indicaendophytic fungiricesymbiotic efficiencynitrogen source