BOR-CHING SHEUWEN-HUNG KUORUEY-JIEN CHENHuang S.-C.KING-JEN CHANGSONG-NAN CHOW2020-01-222020-01-222008https://www.scopus.com/inward/record.uri?eid=2-s2.0-56649094469&doi=10.1016%2fj.breast.2008.06.001&partnerID=40&md5=345a1636c11aef2c78b958a423bfe6fdhttps://scholars.lib.ntu.edu.tw/handle/123456789/452532To investigate the clinical significance of tumor-infiltrating lymphocytes (TILs) within the tumor milieu, we quantitatively measured and compared the subpopulations of TILs in 24 patients with stage I-III breast carcinoma. Peripheral blood mononuclear cells (PBMCs), normal breast parenchyma-infiltrating lymphocytes (NILs), and TILs were isolated from tissue specimens and quantified by flow cytometry. The results showed that increased proportion of CD8+ T cells, with decreased proportion of CD4+ T cells, was significant in gated CD3+ TILs as compared to autologous NILs or PBMCs (P < 0.001). The tumor-infiltrating CD8+ T cells significantly increased with stage progression, reflected in a more strongly decreased CD4/CD8 percentage (P = 0.003). The CD4/CD8 percentage of TILs was strongly correlated with lymphovascular permeation and subsequent lymph node metastasis (P < 0.001). Increased percentages of tumor-infiltrating CD8+ T cells with decreased CD4/CD8 percentages are of prognostic importance for cancer progression in human breast cancer. ? 2008 Elsevier Ltd. All rights reserved.[SDGs]SDG3CD3 antigen; CD4 antigen; CD8 antigen; article; breast carcinoma; cancer growth; cancer staging; CD4 CD8 ratio; CD4+ T lymphocyte; CD8+ T lymphocyte; cell isolation; clinical article; controlled study; flow cytometry; human; human cell; human tissue; lymph node metastasis; lymphocyte subpopulation; peripheral blood mononuclear cell; priority journal; prognosis; quantitative analysis; tumor associated leukocyte; tumor immunityjournal article10.1016/j.breast.2008.06.0012-s2.0-56649094469