2012-08-012024-05-13https://scholars.lib.ntu.edu.tw/handle/123456789/645874摘要：前庭終末受器包括三半規管及二耳石器(橢圓囊及球囊)。床上，半規管功能可經由溫差測驗?檢查，至於球囊功能則藉由氣導音聲?激產生的前庭誘發肌性電位?檢測。後者由於可在持續收縮的頸部肌肉上記?到電位，因此又偁為「頸性」前庭誘發肌性電位。近??發現?用骨導振動?激，前庭誘發肌性電位亦可輕?地在眼外肌記?到電位，謂之「眼性」前庭誘發肌性電位。其反射的?徑乃藉由機械性?激橢圓囊或球囊，進入對側的前庭眼反射，而以肌電位形式表現於眼外肌上。本實驗室在過去已成功地建?天竺鼠之內耳溫差測驗，及「頸性」前庭誘發肌性電位的動物模式。由於前庭誘發肌性電位亦可在眼外肌上取得記?，?能建?「眼性」前庭誘發肌性電位的動物模式，將可對整體前庭終末受器功能之評估，?臻完備。過去，我們曾?用鼓室內投予氨基??藥物，證明?「頸性」前庭誘發肌性電位的發生源自於球囊。進一步配合電子顯微鏡觀察，釐清「頸性」前庭誘發肌性電位的起源?自於球囊斑I 型毛細胞。既然球囊的病態生?學研究，業已釐清，那麼橢圓囊病態生?學的研究，亦刻?容緩。故本三?期計畫包括，第一?計畫?用骨導振動?激源?建?天竺鼠「眼性」前庭誘發肌性電位的動物模式。第二??用gentamicin 注入內耳圓窗膜後，進?「眼性」前庭誘發肌性電位檢查後，予以犧牲，再藉由光學顯微鏡及免疫螢光顯微鏡進?橢圓囊的病?檢查。第三?則同法，但進?電子顯微鏡的檢查。本計畫的最終目的，在建?天竺鼠橢圓囊病變的最佳動物模式。吾人深信這一動物模式的建?，將有助於今後開啟研究橢圓囊病變的新紀元。<br> Abstract: Vestibular endorgans comprise three semicircular canals and two otolithic organs, i.e. thesaccule and utricle. Clinically, semicircular canals can be assessed by caloric and headimpulse tests, whereas the saccule has been examined via air-conducted sound (ACS)stimulation by the vestibular evoked myogenic potential (VEMP) test. The latter can berecorded on tonically contracted cervical muscles, and is termed cervical VEMP (cVEMP). Incontrast, VEMP can also be recorded from extraocular muscles via bone-conducted vibration(BCV) stimuli, which is termed ocular VEMP (oVEMP), and has been referred to the utricularfunction by a crossed vestibulo-ocular reflex.Previously, we have successfully recorded caloric nystagmus (Young et al. 1992) andcVEMPs (Yang and Young 2005) in alert guinea pigs. Applying gentamicin onto the roundwindow membrane of guinea pigs proved that absent cVEMPs are strongly correlated withthe toxic effect of gentamicin on the saccule (Day et al. 2007). Furthermore, electronmicroscopy demonstrated that morphological changes occur mostly in type I hair cellsactivities of the saccular macula (Lue et al. 2009). As the pathophysiology of the sacculardisorder has been thoroughly examined, this study aimed to establish an animal model ofutricular lesions in guinea pigs via physiological and morphological investigation. First year,this study will utilize BCV mode in eliciting oVEMPs in guinea pigs. Second year, usinggentamicin solution dropping onto the round window membrane, we will study themorphological change of the utricle under light and confocal microscopy. Third year, we willperform electron microscopy. Our final goal is to develop an appropriate animal model ofutricular lesion in guinea pigs, which may set the stage to investigate the utricular disorders inthe clinical patients.眼性前庭誘發肌性電位橢圓囊天竺鼠ocular vestibular evoked myogenic potentialutricle